Abstract
Methane-oxidizmg bacteria (methanotrophs) are a unique group of bacteria that grow on methane as then sole source of carbon and energy. They can be isolated from a wide variety of marme and freshwater environments, soils, and sediments and appear to be ubiquitous in the natural environment. They have been classtfred, on the basis of chemotaxonomic studies and 16s rrbosomal RNA phylogenetic analyses, mto five genera: Methylococcus, Methylobacter, Methylomonas, Methylosmus, and Methylocystis (1,2). These five genera fall into two phylogenetically distinct, exclusively methanotrophic groups. Methanotrophs with type I mtracellular membranes include the genera Methylomonas, Methylobacter, and Methylococcus, which are all related to bacterra of the γ-subdivision of the Proteobacteria. Methanotrophs with type II membranes include Methylosinus and Methylocystis, which belong to the α-subdivision of the Proteobacterla (Table 1). There has been considerable interest in methanotrophs since it has been recognized that they are a major sink for atmospheric methane In many natural environments, where these aerobic bacteria are exposed to methane, arising from the biological production by methanogens, they are responsible for removal of much of this methane before it escapes to the atmosphere, and are therefore important in the global carbon cycle (see ref. 3 and chapters therein).
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Bowman, J P., Sly, L I, Nicholas, P D., and Hayward, A C (1993) Revised taxonomy of the methanotrophs description of Methylobacter gen nov., emendanon of Methylococcus, validation of Methylosinus species and a proposal that the family Methylococcaceae includes only group I methanotrophs Int. J. Syst. Bactenol. 43, 735–753
Brusseau, G A., Bulygma, E S., and Hanson, R. S (1994) Phylogenetic analysis and development of probes for differentiatmg methylotrophic bacteria. Appl Environ Microbiol 60, 626–636.
Murrell, J C., and Kelly, D P (1993) Microbial Growth on Cl Compounds, Intercept, Andover, UK.
Murrell, J C. (1994) Molecular genetics of methane oxidation. Biodegradation 5, 145–159
Alvarez-Cohen, L, McCarty, P L, Boulygina, E, Brusseau, G, and Hanson, R. S (1992) Cometabolic biotransformation of trichloroethylene and chloroform by a bacterial consortium grown with methane. Appl Environ. Mzcrobiol. 58, 1886–1893.
Murrell, J C., Holmes, A J., McDonald, I. R., and Kenna, E (1995) The development of molecular ecology techniques for the detection of methanotrophs in the natural environment, in The Microbiology of Atmospheric Trace Gases: Sources, Sinks and Global Change Processes, (Murrell, J C and Kelly, D P., eds ), NATO ASI Series, Springer Verlag, pp 135–151
McDonald, I R., Kenna, E M., and Murrell, J C (1995) Detection of methan-otrophic bacteria in environmental samples by using the polymerase chain reaction. Appl Environ Microblol 61, 116–121
Semrau, J. D., Chlstoserdov, A., Lebron, J., Costello, A., Davagnino, J., Kenna, E, Holmes, A J, Finch, R, Murrell, J. C., and Lidstrom, M E (1995) Particulate methane monooxygenase genes in methanotrophs J Bacterzol 177, 3071–3079.
Bowman, J P (1992) The systematics of methane-utihzmg bacteria PhD Thesis, University of Queensland, Brisbane, Australia
Oakley, C. J., and Murrell, J. C. (1988) nifH genes in obligate methane oxidising bacteria FEMS Mlcroblol. Lett 49, 53–57
Sommerville, C. J., Knight, I T., Straube, W L., and Colwell, R R. (1989) Simple, rapid method for direct isolation of nucleic acids from aquatic environments Appl Environ Microbiol 55, 548–554
Selenska, S., and Klingmuller, W (1991) DNA recovery and direct detection of Tn5 sequences from soil Lett Appl Microbiol 13, 21–24
Bruce, K D., Hiorns, W D., Hobman, J L. Osbom, A M., Stroke, P., and Ritchie, D A (1992) Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction. Appl Environ Microbiol. 58, 3413–3416
Ltesack, W., Weyland, H, and Stackebrandt, E (1991) Potential risks of gene amplication by PCR as determined by 16s rDNA analysis of a mtxed-culture of strict barophilic bacteria Microb Ecol 21, 191–198
Murrell, J C., McGowan, V., and Cardy, D L N (1993) Detection of methy-lotrophic bacteria in natural samples by molecular probing techniques Chemosphere 26, 1–11
Sambrook, J., Frnsch, E F., and Mamatis, T (1989) Transfer of DNA from agarose gels to solid supports, in Molecular Cloning A Laboratory Manual, 2nd ed Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p. 9.34.
Saunders, S E., and Burke, J F (1990) Rapid isolation of miniprep DNA for double strand sequencing Nucleic Acids Res l8, 4948
Amann, R I, Stromley, J., Devereaux, R., Key, R., and Stahl, D A (1992) Molecular and microscopic identiftcatton of sulfate-reducing bacteria in multi-species biofilms Appl Environ Microbiol 58, 614–623
Amann, R I, Ludwig, W., and Schleifer, K H (1995) Phylogenettc identification and in situ detection of individual microbial cells without cultivation Microbiol Rev 59, 143–169
Holmes, A. J., Owens, N J P., and Murrell, J C (1995) Detection of novel marine methanotrophs using phylogenetic and functional gene probes after methane enrichment Microbiology 141, 1947–1955
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© 1997 Humana Press Inc, Totowa, NJ
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McDonald, I.R., Holmes, A.J., Kenna, E.M., Murrell, J.C. (1997). Molecular Methods for the Detection of Methanotrophs. In: Bioremediation Protocols. Methods in Biotechnology™, vol 2. Humana Press. https://doi.org/10.1385/0-89603-437-2:111
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DOI: https://doi.org/10.1385/0-89603-437-2:111
Publisher Name: Humana Press
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