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Analysis of Pentachlorophenol in Soils for Use in Bioremediation Studies

  • Ruth McGrath
  • Ian Singleton
Protocol
Part of the Methods in Biotechnology™ book series (MIBT, volume 2)

Abstract

Pentachlorophenol (PCP) is an envuonmentally persistent chemical that is toxic toward living organisms (1-4) The widespread use of PCP as a general biocide, especially for timber preservation, has led to the contamination of a variety of environments with this chemical (5,6). In particular, soils around wood treatment plants are often contaminated with PCP and bioremediation has been suggested as a useful method in treating pollution of this type (7-9). To carry out a bioremediation process (see Chapter 1), either environmental conditions are optimized, such that indigenous microflora capable of degrading the pollutants are stimulated, or microbial inoculants, known to degrade the chemicals involved, are added to the site (10,11). Central to bioremediation studies is the extraction and accurate analysis of pollutants. The analysis of chemicals in soils is problematic because of the wide variety of interactions that are possible between chemical and soil components. For example, adsorption of PCP to soils is known to be affected by clays (12,13), organic matter (14), and pH (15,16). Furthermore, these interactions are altered by changes in soil moisture (17). Standard methods developed for the recovery of pollutants from soils are timeconsuming and expensive because of the sample preparation required and the methods are not suited to the large number of samples required in bioremediation experiments. Also, workers involved in bioremediation often lack the specialist knowledge required for the efficient analysis of pollutants.

Keywords

Soil Moisture Content High Performance Liquid Chro Soil Moisture Level High Performance Liquid Chro Indigenous Microflora 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Dougherty, R C (1977) Human exposure to pentachlorophenol, in Pentachlorophenol Chemistry, Pharmacology and Environmental Toxicology (Rao, K R, ed.), Plenum, New York, pp 351–361Google Scholar
  2. 2.
    Stephenson, G L., Kaushik, N K., and Solomon, K. R (1991) Chronic toxictty of a pure and technical grade pentachlorophenol to Daphnia magna. Arch Environ. Contam Toxicol 21, 388–394PubMedCrossRefGoogle Scholar
  3. 3.
    Fielder, R J., ed (1982) Pentachlorophenol, Toxicity Review 5, Health and Safety Executive, Baynard’s House, 1 Chepstow Place, London, W2 4TF, UK.Google Scholar
  4. 4.
    Lang, D and Muellerucholtz, W (1991) Human lymphocyte reactivity after in vitro exposure to technical grade and anaytical grade pentachlorophenol. Toxicology 70, 271–283.PubMedCrossRefGoogle Scholar
  5. 5.
    Fountaine, J. E, Joshipura, P B., and Kehher, P. N (1976) Some observations regarding pentachlorophenol levels in Haverford township, Pennsylvania Water Res. 10, 185–188.CrossRefGoogle Scholar
  6. 6.
    Compeau, G C., Mahaffey, W. D., and Patras, L. (1991) Full scale bioremediation of contaminated soil and water, in Environmental Biotechnology for Waste Treatment (Sayler, G. S, ed.) Plenum, New York, pp, 91–109Google Scholar
  7. 7.
    Ceroici, W J and Beresford, C. R (1993) Remediation of an abandoned wood preserving facility in Canada. UNEP Indust Environ. July–Sept., 21–26Google Scholar
  8. 8.
    Kaufman, D. D. (1977) Degradation of pentachlorophenol in soil, and by soil microorganisms, in Pentachlorophenol: Chemistry, Pharmacology and Environmental Toxicology (Rao, K R, ed.), Plenum, New York, pp 27–40Google Scholar
  9. 9.
    Middledorp, P. J M., Briglia, M., and Salkinoja-Salonen, M. S., (1990) Biodegradation of pentachlorophenol in natural soil by inoculated Rhodococcus chlorophenolicus. Microb. Ecol 20, 123–1CrossRefGoogle Scholar
  10. 10.
    Otte, M., Gagnon, J., Comeau, Y., Matte, N., Greer, C W., and Samson, R (1994) Activation of an indigenous microbial consortmm for bioaugmentation of pentachlorophenol/ creosote contaminated soils Appl. Microbiol Biotechnol 40, 926–932.CrossRefGoogle Scholar
  11. 11.
    Aust, S D (1990) Degradation of environmental pollutants by Phanerochaete chrysosporium Microb Ecol. 20, 197–209.CrossRefGoogle Scholar
  12. 12.
    Char, J and Aomine, S (1974) Mechanisms of pentachlorophenol adsorption by soils Soil Sci Plant Nutr. 20, 371–379Google Scholar
  13. 13.
    Xing, B., McGill, B., and Dudas, M. J. (1993) Thermodynamic parameters for pentachlorophenol sorption on montmonllonite in aqueous suspensrons. Chemosphere 26, 1311–1324CrossRefGoogle Scholar
  14. 14.
    Char, J. and Aomme, S (1972) Effects of the soil on the activity of pentachlorophenol Soil Sci Plant Nutr. 18, 255–260Google Scholar
  15. 15.
    Lee, S. L., Rao, P. S., Nkedi-Kizza, P., and Delfino, J J. (1990) Influence of solvent and sorbent characteristics on drstrtbutron of pentachlorophenol in octanolwater and soil-water systems Envir Sci Technol 24, 654–661CrossRefGoogle Scholar
  16. 16.
    Char, J and Aomme, S. (1974) Adsorption of pentachlorophenol by soils Soil Sci Plant Nutr 20, 135–144Google Scholar
  17. 17.
    Wall, A J and Stratton, G W. (1991) Effects of moisture content on the extractability of pentachlorophenol from soil Chemosphere 23, 881–888.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc, Totowa, NJ 1997

Authors and Affiliations

  • Ruth McGrath
    • 1
  • Ian Singleton
    • 2
  1. 1.Department of MicrobiologyUniversity CollegeDublinIreland
  2. 2.Department of Soil ScienceUniversity of AdelaideGlen OsmondAustralia

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