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Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis

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Regulatory Protein Modification

Part of the book series: Neuromethods ((NM,volume 30))

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Abstract

Following translation, many proteins undergo further modifications that can dramatically affect both their physical properties and biological function (Wold and Moldave, 1984; Freedman and Hawkins, 1985; Harding and Crabbe, 1992). These posttranslational modifications are essential to the vitality of all eukaryotic cells, including neurons. Techniques that identify amino acid residues in a given protein that are modified and assess the effect of eliminating a specific modification site on a protein’s function, both in vitro or in the context of cellular expression, are useful in studying posttranslational modifications. The more traditional biochemical and immunological methods of studying posttranslational modification are discussed elsewhere in this book. However, the resources required for these approaches may not always be available or may yield equivocal results. Thus, the powerful tools of molecular biology may provide a viable alternative for identifying sites of posttranslational modification.

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Author information

Authors and Affiliations

  1. Laboratory ofMolecular and Cellular Neuroscience, The Rockefeller University, New York, NY

    James A. Bibb

  2. Institute de Biologia Experimental e Tecnolǵica, Oeiras, Portugal

    Edgar da Cruz e Silva F.

Authors
  1. James A. Bibb
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  2. Edgar da Cruz e Silva F.
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Editor information

Editors and Affiliations

  1. Cornell University Medical College, New York, NY

    Hugh C. Hemmings Jr. (MD,PhD) (MD,PhD)

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Bibb, J.A., da Cruz e Silva F., E. (1997). Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis. In: Hemmings, H.C. (eds) Regulatory Protein Modification. Neuromethods, vol 30. Humana Press. https://doi.org/10.1385/0-89603-415-1:275

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  • DOI: https://doi.org/10.1385/0-89603-415-1:275

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-415-0

  • Online ISBN: 978-1-59259-635-5

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