Abstract
Enzymes can be immobilized near electrode surfaces by trapping them in a crosslinked polymer. Gregg and Heller (1) introduced an mteresting extension of this technique by incorporating the polymer into the sensortransduction mechanism. The strategy was to attach redox complexes to the polymer backbone and use the resultant redox polymer to mediate electron transfer between the immobilized enzymes and the substrate electrode. The principles behind the operation of these enzyme electrodes are essentrally identical to those behind sensors that use soluble mediators. The main difference IS that the redox functionallttes pendant to the crosslinked polymer cannot participate in molecular diffusion, Rather, the electron transport process occurs by a series of self-exchange reactions between neighboring redox centers, a process referred to as electron hopping (2).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gregg, B. A. and Heller, A. (1991) Redox polymer films containing enzymes: 2. Glucose oxidase containing enzyme electrodes. J Phys. Chem 95, 5974–5980.
Dalton, E. F, Surridge, N. A., Jermgan, J. C., Wilbourn, K. O., Facci, J. S., and Murray, R W (1990) Charge transport in electroactive polymers consistmg of fixed molecular redox sites. Chem Phys 141, 143–157.
Garguilo, M. G. and Michael, A. C. (1995) Optimization of amperometrrc microsensors for momtormg cholme in the extracellular fluid of brain trssue Anal Chim Acta 307, 291–299.
O’Hara, T. J, Rajagopalan, R., and Heller, A (1994) “Wired” enzyme electrodes for amperometrtc determmation of glucose or lactate in the presence of interfering substances Anal Chem 66, 2451–2457.
Garguilo, M. G. and Michael, A. C. (1994) Quantitation of choline in the extracellular fluid of brain tissue with amperometric microsensors Anal Chem. 66, 2621–2629.
Lay, P. R., Sargeson, A. M., and Taube, H. (1986) crs-bis(2,2′-Bipyridme-N,N′) complexes of ruthenmm(III)/(II) and osmium(III)/(II), in Inorganic Synthesis, vol. 24 (Shreeve, H. J. M., ed.), Wiley, New York, pp. 291–299.
Gregg, B A and Heller, A. (1991) Redox polymer films contammg enzymes 1. A redox-conductmg epoxy cement synthesis, characterization, and electrocatalytic oxidatron of hydroqumone J Phys Chem 95, 5970–5975
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc.
About this protocol
Cite this protocol
Shankar, L., Garguilo, M.G., Michael, A.C. (1998). Enzyme Biosensors Based on Redox Polymers. In: Mulchandani, A., Rogers, K.R. (eds) Enzyme and Microbial Biosensors. Methods in Biotechnology, vol 6. Humana Press. https://doi.org/10.1385/0-89603-410-0:121
Download citation
DOI: https://doi.org/10.1385/0-89603-410-0:121
Publisher Name: Humana Press
Print ISBN: 978-0-89603-410-5
Online ISBN: 978-1-59259-484-9
eBook Packages: Springer Protocols