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Use of Silver Staining to Detect Nucleic Acids

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Basic DNA and RNA Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 58))

Abstract

Silver stains are useful for the detection of nanogram amounts of proteins or nucleic acids in acrylamide gels or on various membranes. They have been shown to be more sensitive than organic stains in detecting proteins and DNA. They are capable of detecting as little as 0.03 ng/mm2 of DNA (1). In addition, silver stains avoid the mutagenic hazards presented by both ethidium bromide and radioactive detection methods.

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References

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© 1996 Humana Press Inc., Totowa, NJ

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Mitchell, L.G., Bodenteich, A., Merril, C.R. (1996). Use of Silver Staining to Detect Nucleic Acids. In: Harwood, A.J. (eds) Basic DNA and RNA Protocols. Methods in Molecular Biology™, vol 58. Humana Press. https://doi.org/10.1385/0-89603-402-X:97

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  • DOI: https://doi.org/10.1385/0-89603-402-X:97

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-402-0

  • Online ISBN: 978-1-59259-251-7

  • eBook Packages: Springer Protocols

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