Electrophoresis of Sequence Reaction Samples
The underlying principle of DNA sequencing by either the Sanger (1) or Maxam and Gilbert method (2), is the ability to fractionate and resolve long, single-stranded DNA molecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on 1-m long gels (3).
KeywordsPhenol Agar Urea Manganese Chloroform
- 4.Bankier, A. T. and Barrell, B. G. (1983) Shotgun DNA sequencing, in Techniques in Life Sciences, Nucleic Acid Biochemistry vol. B5 (Flavell, R. A., ed.), Elsevier, Ireland, pp. 1–34.Google Scholar
- 6.Bankier, A. T. and Barrell, B. G. (1989) Sequencing single-stranded DNA using the chain termination method, in Nucleic Acids Sequencing: A Practical Approach (Howe, C. J. and Ward, E. S., eds.), IRL, Oxford, pp. 37,38.Google Scholar