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Electrophoresis of Sequence Reaction Samples

  • Alan T. Bankier
Part of the Methods in Molecular Biology™ book series (MIMB, volume 58)

Abstract

The underlying principle of DNA sequencing by either the Sanger (1) or Maxam and Gilbert method (2), is the ability to fractionate and resolve long, single-stranded DNA molecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on 1-m long gels (3).

Keywords

High Voltage Power Supply Saran Wrap Artifact Band Buffer Chamber Film Cassette 
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References

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Alan T. Bankier
    • 1
  1. 1.MRC Laboratory of Molecular BiologyCambridgeUK

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