Electrophoresis of Sequence Reaction Samples
The underlying principle of DNA sequencing by either the Sanger (1) or Maxam and Gilbert method (2), is the ability to fractionate and resolve long, single-stranded DNA molecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on 1-m long gels (3).
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