RNA Slot Blotting

  • David Murphy
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 58)

Abstract

In a slot blot, RNA is applied, unfractionated, to a solid matrix, and therefore, slot blotting is a rapid and sensitive method for analyzing changes in RNA quantity following developmental or physiological changes. Its disadvantage is that it cannot be used when the RNA sample contains additional homologous sequences, which will hybridize to the probe and confuse the results.

Keywords

Formaldehyde Filtration EDTA Nylon Formamide 

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • David Murphy
    • 1
  1. 1.Neuropeptide Laboratory, Institute of Molecular and Cell BiologyNational University of SingaporeRepublic of Singapore

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