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Assay of Neuropeptidases Using Fluorogenic Substrates

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Neuropeptide Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 73))

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References

  1. Schecter, I. and Berger, A. (1968) On the active site of proteases III. Mapping the active site of papain, specific peptide inhibitors of papain. Biochem. Biophys. Res. Commun. 32, 898–902.

    Article  Google Scholar 

  2. Zimmerman, M., Ashe, B., Yurewica, E. C., and Patel, G. (1977) Sensitive assays for trypsin, elastase and chymotrypsm using new fluorogenic substrates. Anal. Biochem. 78, 47–51.

    Article  PubMed  CAS  Google Scholar 

  3. Cornish-Bowden, A. (1995) Fundamentals of Enzyme Kinetics. Portland, London, pp. 58–62.

    Google Scholar 

  4. Storer, A. C. and Cornish-Bowden, A. (1974) The kinetics of coupled enzyme reactions. Biochem. J. 141, 205–209.

    PubMed  CAS  Google Scholar 

  5. Brooks, S. P. J. and Suetler, C. H. (1989) Practical aspects of coupling enzyme theory. Anal. Biochem. 176, 1–14.

    Article  PubMed  CAS  Google Scholar 

  6. Tisljar, U., Knight, C. G., and Barrett, A. J. (1990) An alternative quenched fluorescence substrate for Pz-peptidase. Anal. Biochem. 186, 112–115.

    Article  PubMed  CAS  Google Scholar 

  7. Molineaux, C. J., Yu, B., and Ayala, J. M. (1991) Distribution of endopeptidase 24.15 in rat brain nuclei using a novel fluorogenic substrate: comparison with endopeptidase 24.11. Neuropeptides 18, 49–54.

    Article  PubMed  CAS  Google Scholar 

  8. Knight, C. G. (1991) A quenched fluorescent substrate for thimet peptidase containing a new fluorescent amino acid, DL-2-amino-3-(7-methoxy-4-coumaryl) propionic acid. Biochem. J. 274, 45–48.

    PubMed  CAS  Google Scholar 

  9. Butenas, S., Orfeo, T., Lawson, J. H., and Mann, K. G. (1992) Amino-naphthal-enesulfonamides, a new class of modifiable fluorescent detecting groups and their use in substrates for serine protease enzymes. Biochemistry 31, 5399–5411.

    Article  PubMed  CAS  Google Scholar 

  10. Wang, G. T. and Krafft, G. A. (1992) Automated synthesis of fluorogenic protease substrates: design of probes for Alzheimer’s disease-associated proteases. Bioorganic Med. Chem. Letts. 2, 1665–1668.

    Article  CAS  Google Scholar 

  11. Goudreau, N., Guis, C., Soleilhac, J.-M., and Roques, B. P. (1994) Dns-Gly-p-NO2)Phe-P-Ala, a specific fluorogenic substrate for neutral endopeptidase 24.11. Anal. Biochem. 219, 87–95.

    Article  PubMed  CAS  Google Scholar 

  12. Mumford, R. A., Strauss, A. W., Powers, J. C., Pierzchala, P. A., Nishino, N., and Zimmerman, M. (1980) A zinc metalloprotease associated with dog pancreatic membranes. J. Biol. Chem. 255, 2227–2230.

    PubMed  CAS  Google Scholar 

  13. Mumford, R. A., Pierzchala, P. A., Strauss, A. W., and Zimmerman, M. (1981) Purification of a membrane-bound metalloendopeptidase from porcine kidney that degrades peptide hormones. Proc. Natl. Acad. Sci. USA 78, 6623–6627.

    Article  PubMed  CAS  Google Scholar 

  14. Relton, J. M., Gee, N. S., Matsas, R., Turner, A. J., and Kenny, A. J. (1983) Purification of endopeptidase 24.11 (“enkephalinase”) from pig brain by immuno-adsorbent chromatography. Biochem. J. 215, 519–523.

    PubMed  CAS  Google Scholar 

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© 1997 Humana Press Inc. Totowa, NJ

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Williams, C.H. (1997). Assay of Neuropeptidases Using Fluorogenic Substrates. In: Irvine, G.B., Williams, C.H. (eds) Neuropeptide Protocols. Methods in Molecular Biology™, vol 73. Humana Press. https://doi.org/10.1385/0-89603-399-6:361

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  • DOI: https://doi.org/10.1385/0-89603-399-6:361

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-399-3

  • Online ISBN: 978-1-59259-559-4

  • eBook Packages: Springer Protocols

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