Analysis of Data from “Cold Saturation” Radioligand Binding Experiments

  • William B. Jeffries
  • David Waugh
  • Peter W. Abel
Part of the Methods in Molecular Biology™ book series (MIMB, volume 73)


Nonspecific Binding Ligand Concentration Radioligand Binding Receptor Population Nonlinear Curve Fitting 
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  1. 1.
    Limbird, L. E. (1996) Identification of receptors using direct radioligand binding techniques, in Cell Surface Receptors A Short Course on Theory and Methods, Kluwer Academic, Boston, MA, pp. 61–119.Google Scholar
  2. 2.
    McPherson, G. A. (1986) A mathematical approach to receptor characterization, in Receptor Pharmacology and Function. (Williams, M., Gleenon, R. A., and Timmermans, P. B. M. W. M., eds.), Marcel Dekker, New York, pp. 47–84.Google Scholar
  3. 3.
    DeBlasi, A., O’Reilly, K. O., and Motulsky, H. J. (1989) Calculating receptor number from binding experiments using same compound as radioligand and competitor. Trends Pharmacol. Sci. 10, 227–229.CrossRefGoogle Scholar
  4. 4.
    Cheng, Y. and Prusoff, W. H. (1973) Relationship between inhibition constant (K1) and the concentration of an inhibitor that causes 50% inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 22, 3099–3108.PubMedCrossRefGoogle Scholar
  5. 5.
    Munson, P. J. and Rodbard, D. (1980) LIGAND, a versatile computerized approach for characterization of ligand-binding systems. Anal. Biochem. 107, 220–239.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. Totowa, NJ 1997

Authors and Affiliations

  • William B. Jeffries
    • 1
  • David Waugh
    • 1
  • Peter W. Abel
    • 1
  1. 1.Department of Pharmacology, School of MedicineCreighton UniversityOmaha

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