Preparation of a Membrane Fraction for Receptor Studies and Solubilization of Receptor Proteins with Retention of Biological Activity

  • Mark Wheatley
  • John Howl
  • Nicola J. Yarwood
  • Andrew R. L. Davies
  • Rosemary A. Parslow
Part of the Methods in Molecular Biology™ book series (MIMB, volume 73)

Keywords

Cholesterol Sucrose Phenol Dopamine Rubber 

References

  1. 1.
    Helenius, A. and Simons, K. (1975) Solubilization of membranes by detergents. Biochim. Biophys. Acta 415, 213–279.Google Scholar
  2. 2.
    Tanford, C. and Reynolds, J. A. (1976) Characterization of membrane proteins in detergent solutions. Biochim. Biophys. Acta. 457, 133–170.PubMedGoogle Scholar
  3. 3.
    Helenius, A., McCaslin, D. R., Friess, E., and Tanford, C. S. (1979) Properties of detergents. Methods Enzymol. 56, 734–749.PubMedCrossRefGoogle Scholar
  4. 4.
    Hjelmeland, L. M. and Chrambach, A. (1981) Electrophoresis and electrofocusing in detergent-containing media: a discussion of concepts. Electrophoresis. 2, 1–11.CrossRefGoogle Scholar
  5. 5.
    Kirilovsky, J. and Schramm, M. (1983) Delipidation of a β-adrenergic receptor preparation and reconstitution by specific lipids. J. Biol. Chem. 258, 6841–6849.PubMedGoogle Scholar
  6. 6.
    Anholt, R., Fredkin, D. R., Deerinck, T., Ellisman, M., Montal, M., and Lindstrom, J. (1982) Incorporation of acetylcholine receptors into liposomes. J. Biol. Chem. 257, 7122–7134.PubMedGoogle Scholar
  7. 7.
    Simonds, W. F., Koski, G., Streaty, R. A., Hjelmland, L. M., and Klee, W. A. (1980) Solubilization of active opiate receptors. Proc. Natl. Acad. Sci. USA 77, 4623–4627.PubMedCrossRefGoogle Scholar
  8. 8.
    Sigel, E. and Barnard, E. A. (1984) A γ-ammobutyric acid/benzodiazepine receptor complex from bovine cerebral cortex. J. Biol. Chem. 259, 7219–7223.PubMedGoogle Scholar
  9. 9.
    Poyner, D. R., Birdsall, N. J. M., Curtis, C. A. M., Eveleigh, P., Hulme, E. C., Pedder, E. K., and Wheatley, M. (1989) Binding and hydrodynamic properties of muscarinic receptor subtypes solubilized in 3-(3-cholamidopropyl) dimethylammonio-2-hydroxy-1-propane sulfonate. Mol. Pharmacol. 36, 420–429.PubMedGoogle Scholar
  10. 10.
    Wheatley, M., Hall, J. M., Frankham, P. A., and Strange, P. G. (1984) Improvement in conditions for solubilization and characterisation of brain D2 dopamine receptors using various detergents. J. Neurochem. 43, 926–934.PubMedCrossRefGoogle Scholar
  11. 11.
    Sigel, E., Stephenson, F. A., Mamalaki, C., and Barnard, E. A. (1983) A γ-amino-butyric acid/benzodiazepine receptor complex of bovine cerebral cortex. J. Biol. Chem. 258, 6965–6971.PubMedGoogle Scholar
  12. 12.
    Berrie, C. P, Birdsall, N. J. M., Haga, K., Haga, T., and Hulme, E. C. (1984) Hydrodynamic properties of muscarinic acetylcholme receptors solubilized from rat forebrain. Brit. J. Pharmacol. 82, 839–851.Google Scholar
  13. 13.
    Peterson, G. L., Rosenbaum, L. C., and Schimerlik, M. I. (1988) Solubilization and hydrodynamic properties of pig atrial muscarinic acetylcholine receptor in dodecyl β-D-maltoside. Biochem. J. 255, 553–560.PubMedGoogle Scholar
  14. 14.
    Wheatley, M. and Strange, P. G. (1983) Characterisation of brain D2 dopamine receptors solubilized by lysophosphatidylcholine. FEBS Letts. 151, 97–101.CrossRefGoogle Scholar
  15. 15.
    Panayotou, G. N., Magee, A. I., and Geisow, M. J. (1985) Reconstitution of epidermal growth factor receptor in artificial lipid bilayers. FEBS Letts. 183, 321–325.CrossRefGoogle Scholar
  16. 16.
    Gonzalez-Ros, J. M., Paraschos, A., and Martinez-Carrion, M. (1980) Reconstitution of functional membrane-bound acetylcholine receptor from isolated torpedo californica receptor protein and electroplax lipid. Proc. Natl. Acad. Sci. USA 77, 1796–1800.PubMedCrossRefGoogle Scholar
  17. 17.
    Franklin, G. I. and Potter, L. T. (1972) Studies of the binding of α-bungarotoxin to membrane-bound and detergent-dispersed acetylcholine receptors from torpedo electric tissue. FEBS Letts. 28, 101–106.CrossRefGoogle Scholar
  18. 18.
    Peterson, G. L., Rosenbaum, L. C., Broderick, D. J., and Schimerlik, M. I. (1986) Physical properties of the purified cardiac muscarinic acetylcholine receptor. Biochemistry 25, 3189–3202.PubMedCrossRefGoogle Scholar
  19. 19.
    Wheatley, M. (1983) The Isolation, characterisation and reconstitution of dopamine receptors from bovine caudate nucleus. Ph.D thesis, University of Nottingham.Google Scholar
  20. 20.
    Klein, U. and Fahrenholz, F. (1994) Reconstitution of the myometrial oxytocin receptor into proteoliposomes. Eur. J. Biochem. 220, 559–567.PubMedCrossRefGoogle Scholar
  21. 21.
    Tiller, G. E., Mueller, T. J., Dockter, M. E., and Struve, W. G. (1984) Hydrogenation of Triton X-100 eliminates its fluorescent and ultraviolet light absorption while preserving its detergent properties. Anal. Biochem. 141, 262–266.PubMedCrossRefGoogle Scholar
  22. 22.
    Hjelmeland, L. M. (1980) A non-denaturing zwitterionic detergent for membrane biochemistry: design and synthesis. Proc. Natl. Acad. Sci. USA 77, 6368–6370.PubMedCrossRefGoogle Scholar
  23. 23.
    Hjelmeland, L. M., Nebert, D. W., and Osborne, J. C. (1983) Sulphobetaine of bile acids: nondenaturing surfactants for membrane proteins. Anal. Biochem. 130, 72–82.PubMedCrossRefGoogle Scholar
  24. 24.
    Repke, H. (1987) Muscarinic receptor-detergent complexes with different biochemical properties—selective solubilization, lectin affinity chromatography and ligand binding studies. Biochim. Biophys. Acta. 929, 47–61.PubMedCrossRefGoogle Scholar
  25. 25.
    Ashani, Y. and Catravas, G. N. (1980) Highly reactive impurities in Triton X-100 and Brij 35: partial characterization and removal. Anal. Biochem. 109, 55–62.PubMedCrossRefGoogle Scholar
  26. 26.
    Rivnay, B. and Metzger, H. (1982) Reconstitution of the receptor for immunoglobulin E into liposomes. J. Biol. Chem. 257, 12,800–12,808.PubMedGoogle Scholar
  27. 27.
    Hjelmeland, L. M. and Chrambach, A. (1984) Solubilization of functional membrane proteins. Methods Enzymol. 104, 305–318.PubMedCrossRefGoogle Scholar
  28. 28.
    Berrie, C. P., Birdsall, N. J. M., Dadi, H. K., Hulme, E. C., Morris, R. J., Stockton, J. M., and Wheatley, M. (1985) Purification of the muscarinic acetylcholine receptor from rat forebrain. Biochem. Soc. Trans. 13, 1101–1103.PubMedGoogle Scholar
  29. 29.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with a folin phenol reagent. J. Blol. Chem. 193, 265–275.Google Scholar
  30. 30.
    Dulley, J. R. and Grieve, P. A. (1975) A simple technique for eliminating interference by detergents in the Lowry method of protein determination. Anal Biochem 64, 136–141.PubMedCrossRefGoogle Scholar
  31. 31.
    Gorissen, H., Aerts, G., Ilien, B., and Laduron, P. (1981) Solubilization of muscarinic acetylcholine receptor from bovine brain: An analytical approach. Anal. Biochem. 111, 33–41.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. Totowa, NJ 1997

Authors and Affiliations

  • Mark Wheatley
    • 1
  • John Howl
    • 1
  • Nicola J. Yarwood
    • 1
  • Andrew R. L. Davies
    • 1
  • Rosemary A. Parslow
    • 1
  1. 1.Department of BiochemistryUniversity of BirminghamUK

Personalised recommendations