Advertisement

Tritium Labeling of Neuropeptides

  • Géza Tóth
  • Sándor Lovas
  • Ferenc Ötvös
Part of the Methods in Molecular Biology™ book series (MIMB, volume 73)

Keywords

Specific Radioactivity Precursor Peptide Label Amino Acid Isotope Exchange Reaction Tritium Atom 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Wilzbach, K. E (1957) Tritium-labelling by exposure of organic compounds to tritium gas. J. Am. Chem. Soc. 79, 1013.CrossRefGoogle Scholar
  2. 2.
    Oehlke, J., Mittag, E., Tóth, G., Bienert, M., and Niedrich, H (1987) Enhanced incorporation of nonhydrolyzable tritium in GnRH and TRF by catalytic exchange labeling. J. Labelled Compd. Radiopharm. 24, 1483–1491.CrossRefGoogle Scholar
  3. 3.
    Zolotarev, Y. A., Kozik, V. S., Dorokhova, E. M., and Myasoedov, N. F. (1991) The investigation of solid-state catalytic hydrogenation of organic compounds. II The synthesis of tritium-labelled valine. J. Labelled Compd. Radiopharm. 24, 997–1007.CrossRefGoogle Scholar
  4. 4.
    Cooper, D and Reich, E (1972) Neurotoxin from venom of cobra, Naja siamensis J. Biol. Chem. 247, 3008–3013.PubMedGoogle Scholar
  5. 5.
    Nagren, K., Franzé, H. M., Ragnarsson, U., and Langstrom, B. (1988) Synthesis of two S-(methyl-3H)-labelled enkephalins and S-(methyl-14C) substance P. J. Labelled Compd. Radiopharm. 25, 141–148.CrossRefGoogle Scholar
  6. 6.
    Hellio, F., Lecocq, G., Morgat, J. L., and Gueguen, P. (1990) Total synthesis of fully tritiated leu-enkephalin by enzymatic coupling J. Labelled Compd. Radiopharm. 28, 991–999.CrossRefGoogle Scholar
  7. 7.
    Morgat, J. L. and Fromageot, P. (1973) Preparation of tritium-labelled peptide hormones of high specific radioactivity, in Radiopharmaceuticals and Labelled Compounds II. IAEA-SM-171/60, Vienna, 109–119.Google Scholar
  8. 8.
    Teplán, I., Mezô, I., Nikolics, K., Seprôdi, J., Kéri, Gy., Bienert, M., and Klauschenz, E. (1982) Tritium Labeling of Brain peptides, in Hormonally Active Brain Peptides. (McKerns, K. W. and Pantic, V., eds.), Plenum, New York, pp. 599–618.Google Scholar
  9. 9.
    Fraker, P. J. and Speck, J. C. (1978) Protein and cell membrane iodinations with soluble chloroamide 1,3,4,6-tetrachloro-3a,6a-diphenilglycolouril. Biochem. Biophysical Res. Comm. 80, 849–857.CrossRefGoogle Scholar
  10. 10.
    Brundish, D. E. and Wade, R. (1973) Synthesis of [3,5-3H2-Tyr23]-α-corticotrophin-(1-24)-tetracosapeptide. J. Chem. Soc. Perkin Trans 1, 2875–2879.CrossRefGoogle Scholar
  11. 11.
    Bienert, M., Klauschenz, E., Nikolics, K., and Niedrich, H. (1981) Protection of methionine in peptides during iodination by sulfonium salt formation Int. J. Pept. Protein Res. 19, 310–314.CrossRefGoogle Scholar
  12. 12.
    Morgat, J. L., Girma, J. P., and Fromageot, P. (1977) Tritium labelling of peptidic hormones First International Symposium on Hormonal Receptors in Digestive Tract Physiology, INSERM Symp. No. 3. p. 43, Elsevier, Amsterdam.Google Scholar
  13. 13.
    Fellion, E., Gacel, G., Roques, B. P., Roy, R., and Morgat, J. L. (1990) Tritium labelling of highly selective probes for δ-opioid receptors. J. Labelled Compd. Radiopharm. 28, 867–876.CrossRefGoogle Scholar
  14. 14.
    Hartrodt, B., Tóth, G., Neubert, K., Sirokmán, F., Baláspiri, L., and Schulz, H. (1983) Synthesis of 14C-and 3H-labelled β-casomorphin-5. J. Labelled Compd. Radiopharm. 20, 39–52.CrossRefGoogle Scholar
  15. 15.
    Nevin, S. T., Kabasakal, L., Otvos, F., Tóth, G., and Borsodi, A. (1994) Binding characteristics of the novel highly selective delta agonist, [3H-Ile5,6]deltorphin II. Neuropeptides 26, 261–265.PubMedCrossRefGoogle Scholar
  16. 16.
    Hasegawa, H., Shinohara, Y., and Baba, S. (1991) Synthesis of 3H-labelled enkephalins for metabolic and pharmacokinetic studies, in Synthesis and Applications of Isotopically Labelled Compounds (Buncel, E and Kabalka, G. W., eds.), Elsevier, Amsterdam, pp 486–489.Google Scholar
  17. 17.
    Búzas, B., Tóth, G., Cavagnero, S., Hruby, V. J., and Borsodi, A. (1992) Synthesis and binding characteristics of the highly delta-specific new tritiated opioid peptide [3H]deltorphin II. Life Sciences 50, PL 75–78.CrossRefGoogle Scholar
  18. 18.
    Shu, A. Y. L. and Heys, R. (1991) Synthesis of a tritiated growth hormone releasing peptide, in Synthesis and Application of Isotopically Labelled Compounds. (Buncel, E. and Kabalka, G. W., eds.), Elsevier, Amsterdam, pp 85–88.Google Scholar
  19. 19.
    Hsi, R. S. P., Stolle, W. T., and Bundy, G. L. (1994) Synthesis of tritium labeled renin inhibitor ditekiren. J. Labelled Compd. Radiopharm. 24, 1175–1186.CrossRefGoogle Scholar
  20. 20.
    Janáky, T., László, F. A., and Morgat, J.-L. (1984) Tritiation of vasopressin analogues and their metabolic fate after intravenous injection in the rat. Acta Medica Hungarica 41(1), 43–54.PubMedGoogle Scholar
  21. 21.
    Tóth, G., Gulyás, É., and Kéri, Gy., unpublished results.Google Scholar
  22. 22.
    Aharony, D., Catanese, C. A., and Woodhouse, D. P. (1991) Binding of the novel ligand [4,5-3H-Leu10]substance P to high-affinity NK-1 receptors on guinea pig lung membranes: modulation by GTP analogs and sulfhydryl modifying agents. J. Pharm. Exp. Ther. 229, 146–155.Google Scholar
  23. 23.
    Aharony, D., Conner, G. E., and Woodhouse, D. P. (1992) Pharmacologic characterization of the novel ligand [4,5-3H-Leu9]neurokinin-A binding to NK-2 receptors on hamster urinary bladder membranes. Neuropeptides 23, 121–130.PubMedCrossRefGoogle Scholar
  24. 24.
    Eberle, A. N., Siegrist, W., Drozdz, R., Verm, V. J., Bagutti, C., Solca, F., Gerard, J., and Zeller, A. (1991) Radiolabelled melanotropic peptides (α-MSH and MCH) for receptor identification, in Synthesis and Application of Isotopically Labelled Compounds (Buncel, E. and Kabalka, G. W., eds.), Elsevier, Amsterdam, pp. 110–115.Google Scholar
  25. 25.
    Felix, A. M., Wang, C. T., Liebman, A. A., Delaney, C. M., Mowles, T., Burghardt, B., Charnecki, A. M., and Metenhofer, J. (1977) Synthesis, biological activity and tritiation of L-3,4-dehydroproline-containing peptides Int. J. Pept. Protein Res. 10, 299.PubMedCrossRefGoogle Scholar
  26. 26.
    Tóth, G. and Strokmán, F. (1981) Külonféle 3H-jelolési módszerek, in Izotóptechnika (Budapest) 24, 259–267.Google Scholar

Copyright information

© Humana Press Inc. Totowa, NJ 1997

Authors and Affiliations

  • Géza Tóth
    • 1
  • Sándor Lovas
    • 2
  • Ferenc Ötvös
    • 1
  1. 1.Biological Research Center of the Hungarian Academy of SciencesSzegedHungary
  2. 2.Department of Biomedical Sciences, School of MedicineCreighton UniversityOmaha

Personalised recommendations