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Amino Acid Sequencing of Neuropeptides

  • Ka Wan Li
  • Wijnand P. M. Geraerts
Part of the Methods in Molecular Biology™ book series (MIMB, volume 73)

Abstract

The determination of the primary structure of peptides to-date has usually been carried out by automated amino acid sequencing involving Edman chemistry. This sensitive method is reliable, easy to perform, and the interpretation of the results is straightforward. However, other techniques such as mass spectrometry and enzymatic degradation are needed to confirm the sequence and/or to detect the posttranslational modifications. In this chapter, we will focus on the Edman degradation method, and describe the complementary methods as judged appropriate.

Keywords

High Performance Liquid Chromatography Reaction Chamber Glass Fiber Filter Edman Degradation Amino Acid Sequencing Data 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. Totowa, NJ 1997

Authors and Affiliations

  • Ka Wan Li
    • 1
  • Wijnand P. M. Geraerts
    • 1
  1. 1.Faculteit der BiologieVrije UniversiteitAmsterdamThe Netherlands

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