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Protein Synthesis pp 365–378Cite as

Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5′ or 3′ UTRs of Translationally Regulated mRNAs

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 77))

Abstract

Numerous recent studies have reported the identification of regulatory sequences located within either the 5′ or 3′ untranslated regions (UTRs) in individual mRNAs which are known to be controlled at the translational level (for review, see ref. 1). It is generally presumed in these cases that the observed mRNA-specific translational regulation is mediated by protein(s) interacting with these sequences. The techniques of gel retardation (also known as band shift or gel mobility shift) and UV crosslinking have been particularly instrumental in the mitial studies to detect the putative regulatory factors.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, UK

    James Walker & Nancy Standart

  2. Department of Biochemistry and Molecular Biology, University of Miami, FL

    Osvaldo de Melo Neto

Authors
  1. James Walker
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  2. Osvaldo de Melo Neto
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  3. Nancy Standart
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Editor information

Editors and Affiliations

  1. Blond McIndoe Center, Queen Victoria Hospital, Sussex, England

    Robin Martin

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© 1998 Humana Press Inc.

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Walker, J., de Melo Neto, O., Standart, N. (1998). Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5′ or 3′ UTRs of Translationally Regulated mRNAs. In: Martin, R. (eds) Protein Synthesis. Methods in Molecular Biology, vol 77. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-397-X:365

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  • DOI: https://doi.org/10.1385/0-89603-397-X:365

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-397-9

  • Online ISBN: 978-1-59259-563-1

  • eBook Packages: Springer Protocols

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