Abstract
Synthetic tRNAs—those that are transcribed in vitro from a DNA sequence that has been ligated into an appropriate plasmid—have a wide variety of applications that range from testing tRNAs for their requirements for amino-acylation (1) to providing reagents for the investigation of protein folding (2). The examples that will be described here involve specific tRNAs with altered anticodons. The construction of several of these tRNAs will be given in detail as examples of the procedure applied. Also, methods of use and how to test the efficiency of these tRNAs will be reviewed.
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Kramer, G., Kudlicki, W., Hardesty, B. (1998). In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons. In: Martin, R. (eds) Protein Synthesis. Methods in Molecular Biology, vol 77. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-397-X:105
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DOI: https://doi.org/10.1385/0-89603-397-X:105
Publisher Name: Springer, Totowa, NJ
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