A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors
The supplemented rabbit reticulocyte lysate is one of the most active cell-free translation systems known and has been extensively used over the last 20 yr or so for in vitro translation of endogenous (globin), as well as of heterologous mRNAs. In addition, it has regularly served as the starting material for the purification, identification, and indeed the characterization of some of the many protein factors required for eukaryotic protein synthesis.
KeywordsInitiation Factor Translation System Affinity Matrix Rabbit Reticulocyte Lysate Translation Assay
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