A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors

  • Michael Rau
  • Theophile Ohlmann
  • Virginia M. Pain
  • Simon J. Morley
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 77)

Abstract

The supplemented rabbit reticulocyte lysate is one of the most active cell-free translation systems known and has been extensively used over the last 20 yr or so for in vitro translation of endogenous (globin), as well as of heterologous mRNAs. In addition, it has regularly served as the starting material for the purification, identification, and indeed the characterization of some of the many protein factors required for eukaryotic protein synthesis.

Keywords

Glycerol Titration Sedimentation MgCl2 Fractionation 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Michael Rau
    • 1
  • Theophile Ohlmann
    • 1
  • Virginia M. Pain
    • 1
  • Simon J. Morley
    • 1
  1. 1.Biochemistry Laboratory, School of Biological SciencesUniversity of SussexBrightonUK

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