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Lectin Methods and Protocols pp 175–192Cite as

Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 9))

Abstract

The existence of O-linked glycans in viral glycoproteins was described in the early 1980s for enveloped viruses such as herpes simplex virus type 1 (HSV-1), vaccinia virus, and mouse hepatitis virus (1-4). Glycoprotein C of HSV-1 (designated gC-1) was demonstrated to contain domains, in which numerous O-linked glycans were concentrated to pronase-resistant clusters (5-7), thereby resembling the organization of mucins (8). Thus glycoprotein, containing nine sites for N-linked glycosylation in addition to the O-linked glycans, is responsible for several important biological activities, including virus receptor binding (9) and binding of factor C3b of the complement system (10). The function of the O-linked glycans in these activities remains unclear, but it is conceivable that their clustered appearance may cause gC-1 to adopt an extended fibrous conformation (11), as originally demonstrated for the O-linked glycans of mucins (8). Use of lectins facilitates a structural analysis of clustered O-linked glycans of gC-1 and it is possible that the methodology presented here may be of more general use, as similar arrangements of clustered O-linked glycans are present in an increasing number known glycoproteins of other enveloped viruses including herpes simplex virus type 2 (12,13), Epstein-Barr virus (14), and respiratory syncytial virus (15).

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Authors and Affiliations

  1. Department of Clinical Virology, University of Göteborg, Guldhedsgatan, Göteborg, Sweden

    Sigvard Olofsson & Anders Bolmstedt

Authors
  1. Sigvard Olofsson
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  2. Anders Bolmstedt
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Editors and Affiliations

  1. University of Liverpool, UK

    Jonathan M. Rhodes  & Jeremy D. Milton  & 

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© 1998 Humana Press Inc.

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Olofsson, S., Bolmstedt, A. (1998). Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins. In: Rhodes, J.M., Milton, J.D. (eds) Lectin Methods and Protocols. Methods in Molecular Medicine™, vol 9. Humana Press. https://doi.org/10.1385/0-89603-396-1:175

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  • DOI: https://doi.org/10.1385/0-89603-396-1:175

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-396-2

  • Online ISBN: 978-1-59259-593-8

  • eBook Packages: Springer Protocols

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