Detection of Altered Glycosylation of α-Fetoprotein Using Lectin-Affinity Electrophoresis

  • Kazuhisa Taketa
  • Miao Liu
  • Hiroko Taga
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 9)

Abstract

α-Fetoprotein (AFP) is an oncofetal glycoprotein with a molecular mass of 70,000 Dalton and a carbohydrate content of approx 4%, i.e., one carbohydrate chain per molecule. High concentrations of AFP are found in the fetal serum as well as in amniotic fluid. Serum concentration of AFP in cord blood at birth range from 10,000–100,000 ng/mL, and it becomes suppressed to levels below 10 ng/mL by 300 d after birth (1). AFP is re-expressed frequently in hepatocellular carcinomas and yolk sac tumors, and much less frequently in gastrointestinal carcinomas.

Keywords

Phenol Foam Agarose Mold Electrophoresis 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Kazuhisa Taketa
    • 1
  • Miao Liu
    • 1
  • Hiroko Taga
    • 1
  1. 1.Department of Public HealthOkayama University Medical SchoolOkayamaJapan

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