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PRINS and In Situ PCR Protocols pp 39–44Cite as

Multiple Sequential Oligonucleotide Primed In Situ DNA Syntheses (MULTI-PRINS)

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 71))

Abstract

Conventional PRINS (if it is possible to use such a description for a relatively new technique) is capable of identifying and quantifying chromosomes or chromosome pairs in metaphase or interphase cells (1–4). Each PRINS reaction can only identify one pair of homologous chromosomes, because the nature of the reaction means that the product of only one primer or primer pair can be specifically labeled in each reaction. However, by inserting a blocking step after each PRINS reaction to ensure that the 3′-ends of the products of the previous reaction cannot act as primers for the next reaction, it is possible to perform several PRINS reactions on a single slide, and therefore ascertain the number of each of several pairs of chromosomes present in a given sample.

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References

  1. Koch, J. E., Kolvraa, S., Petersen, K. B., Gregersen, N., and Bolund, I. (1989) Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ. Chromosoma 98, 259–265.

    Article  PubMed  CAS  Google Scholar 

  2. Gosden, J., Hanratty, D., Starling, J., Fantes, J., Mitchell, A., and Porteous, D. (1991) Oligonucleotide primed in situ DNA synthesis (PRINS): a method for chromosome mapping, banding and investigation of sequence organization. Cytogenet. Cell Genet. 57, 100–104.

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  3. Gosden, J. and Lawson, D. (1994) Rapid chromosome identification by oligonucleotide primed in situ DNA synthesis (PRINS). Hum. Mol. Genet. 3, 931–946.

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  4. Gosden, J. and Lawson, D. (1995) Instant PRINS: a rapid method for chromosome identification by detecting repeated sequences in situ. Cytogenet. Cell Genet. 68, 57–60.

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Author information

Authors and Affiliations

  1. Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, Scotland

    John R. Gosden & Diane Lawson

Authors
  1. John R. Gosden
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  2. Diane Lawson
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Editor information

Editors and Affiliations

  1. Western General Hospital, Edinburgh, UK

    John R. Gosden

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© 1997 Humana Press Inc, Totowa, NJ

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Gosden, J.R., Lawson, D. (1997). Multiple Sequential Oligonucleotide Primed In Situ DNA Syntheses (MULTI-PRINS). In: Gosden, J.R. (eds) PRINS and In Situ PCR Protocols. Methods in Molecular Biology™, vol 71. Humana Press. https://doi.org/10.1385/0-89603-395-3:39

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  • DOI: https://doi.org/10.1385/0-89603-395-3:39

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-395-5

  • Online ISBN: 978-1-59259-557-0

  • eBook Packages: Springer Protocols

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