In Situ PCR Amplification of Intracellular mRNA

  • Raymond H. Chen
  • Susan V. Fuggle
Part of the Methods in Molecular Biology™ book series (MIMB, volume 71)


The polymerase chain reaction (PCR) is now commonly used in laboratories involved in research studies and clinical diagnostic work (1,2). A major advantage of PCR combined with reverse transcription (RT-PCR) is that it can be used to amplify and detect rare mRNA within a specimen. However, conventional RT-PCR cannot be used either to quantitate the frequency of cells expressing a particular mRNA or determine the cellular origin of the amplified signal. Both of these factors may be relevant in the interpretation of gene expression.


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Copyright information

© Humana Press Inc, Totowa, NJ 1997

Authors and Affiliations

  • Raymond H. Chen
    • 1
  • Susan V. Fuggle
    • 1
  1. 1.Nuffield Department of SurgeryJohn Radcliffe HospitalHeadingtonUK

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