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In Situ PCR Amplification of Intracellular mRNA

  • Raymond H. Chen
  • Susan V. Fuggle
Part of the Methods in Molecular Biology™ book series (MIMB, volume 71)

Abstract

The polymerase chain reaction (PCR) is now commonly used in laboratories involved in research studies and clinical diagnostic work (1,2). A major advantage of PCR combined with reverse transcription (RT-PCR) is that it can be used to amplify and detect rare mRNA within a specimen. However, conventional RT-PCR cannot be used either to quantitate the frequency of cells expressing a particular mRNA or determine the cellular origin of the amplified signal. Both of these factors may be relevant in the interpretation of gene expression.

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Product Aluminum Foil Cytospin Preparation Tube Polymerase Chain Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc, Totowa, NJ 1997

Authors and Affiliations

  • Raymond H. Chen
    • 1
  • Susan V. Fuggle
    • 1
  1. 1.Nuffield Department of SurgeryJohn Radcliffe HospitalHeadingtonUK

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