The Preparation and Use of Brain Slices and Dissociated Neurons for Patch-Clamp Studies of Neurotransmitter Action
The development of in vitro brain slice and isolated neuron techniques has greatly facilitated detailed studies of the electrophysiology of a wide range of neuronal types in the adult and neonatal vertebrate central nervous system (CNS). Particularly advantageous are the greater mechanical stability that these preparations provide over in vivo models and the control allowed over the composition of the extracellular environment. In addition, the development of the patch-clamp technique has opened up the possibility of direct access to the intracellular environment via internal patch pipet solutions. In combination, these approaches have enabled detailed investigations of neuronal membrane properties, the cellular actions of neurotransmitters, and synaptic mechanisms.
KeywordsBrain Slice Razor Blade Incubation Chamber Cell Dissociation Cyanoacrylate Glue
- 6.Hećimović, H., Rusin, K. I., Ryu, P. D., and Randic, M. (1990) Modulation of excitatory amino-acid induced currents by tachykinins and opioid peptides in rat spinal cord neurons. Soc. Neurosci. Abstr. 16, 853.Google Scholar
- 8.Lawrence, J., Penington, N. J., and Kelly J. S., (1989) Identification of acutely dissociated neurons from the slices of adult rat brainstem as projecting neurons of the Nucleus Raphe Dorsalis (NRD). Neurosci. Lett. 36(Suppl.), 99.Google Scholar