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The Preparation and Use of Brain Slices and Dissociated Neurons for Patch-Clamp Studies of Neurotransmitter Action

  • Philip M. Larkman
  • Hrvoje Hećimović
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 72)

Abstract

The development of in vitro brain slice and isolated neuron techniques has greatly facilitated detailed studies of the electrophysiology of a wide range of neuronal types in the adult and neonatal vertebrate central nervous system (CNS). Particularly advantageous are the greater mechanical stability that these preparations provide over in vivo models and the control allowed over the composition of the extracellular environment. In addition, the development of the patch-clamp technique has opened up the possibility of direct access to the intracellular environment via internal patch pipet solutions. In combination, these approaches have enabled detailed investigations of neuronal membrane properties, the cellular actions of neurotransmitters, and synaptic mechanisms.

Keywords

Brain Slice Razor Blade Incubation Chamber Cell Dissociation Cyanoacrylate Glue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1997

Authors and Affiliations

  • Philip M. Larkman
    • 1
  • Hrvoje Hećimović
    • 1
  1. 1.Department of PharmacologyUniversity of EdinburghScotland

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