Abstract
Proteins III and VIII of filamentous phage coat (1) have been reported as suitable for the construction of phage-displayed peptide libraries (2–4). Protein VIII (pVII1) is the major coat protein, present in approx 2700 copies per phage particle, and its amino-terminus tolerates insertions of up to six amino acids (5–7). Larger peptide inserts can be displayed only when a two-gene system is used (6,8), thus obtaining hybrid phage particles containing both recombinant and wild-type proteins. The high copy number of recombinant pVIII molecules per phage particle provides a highly sensitive system, and linear and constrained peptide libraries in pVIII have been successfully used for the selection of specific ligands for several different monoclonal and polyclonal antibodies (9).
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© 1998 Humana Press Inc., Totowa, NJ
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Luzzago, A., Felici, F. (1998). Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIII. In: Cabilly, S. (eds) Combinatorial Peptide Library Protocols. Methods in Molecular Biology™, vol 87. Humana Press. https://doi.org/10.1385/0-89603-392-9:155
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DOI: https://doi.org/10.1385/0-89603-392-9:155
Publisher Name: Humana Press
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