Preparation of Biocompatible Resins for Library Syntheses

  • Morten Meldal
Part of the Methods in Molecular Biology™ book series (MIMB, volume 87)


The open structure of the biocompatible polyethylene glycol polyamide copolymer (PEGA resin) is presented in Fig. 1 , exemplified by the application of the commercially available bis-2-aminopropyl-PEG1900. With the use of this PEG1900, permeability with proteins up to 50 kDa has been achieved, as demonstrated by gel permeation chromatography (1). A resin-bound fluoroescencequenched peptide substrate showed 80% cleavage with subtihsin Carlsberg (MW 27 kDa) in 1 h and the cleavage went to completion in ∼2 h. The resin could also be used for glycopeptide assembly using bovine β-(1→4)-galactosyl transferase (MW 43/49 kDa) to transfer galactose to the 4-position of GlcNAc (2) In this example, the diffusion and reorientation of the enzyme inside the polymer network was a rate-limiting factor for the reaction, which could, however, be brought to completion in 72 h This indicates that the reaction was performed at the practical limit of protein size for preparative enzyme reactions in this resin. PEGA resins perform excellently in solid-phase assays of biomolecular reactions. Furthermore, they are transparent and no light is absorbed above 250 nm, so they can be used with a variety of different chromophores and fluorescent probes for detection of biomolecular reactions.
Fig. 1.

Preparation of PEGA resin containing PEG1900 by the partial acryloylation procedure.


Suspension Polymerization Tetramethyl Ethylene Diamine Acryloyl Chloride Zero Gravity Sorbitan Monolaurate 
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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Morten Meldal
    • 1
  1. 1.Department of ChemistryCarlsberg LaboratoriesCopenhagenDenmark

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