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Bacterial and Coelenterate Luciferases as Reporter Genes in Plant Cells

  • Protocol
Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 82))

  • 1997 Accesses

Abstract

The study of gene regulation has been greatly enhanced by the use of reporter gene systems such as beta galactosidase (P-gal), neomycin phosphotransferase (APH[3]11), chloramphenicol acetyl transferase (CAT), beta glucuronidase (GUS), and dihydrofolate reductase (DHFR). In the past several years, development of marker gene systems based on bioluminescence have extended the power of marker gene technology from enzymatic or color based in vitro assays to more sensitive single photon counting methods m vitro and in vivo A variety of proteins that catalyze bioluminescent reactions have been isolated and characterized. For several of these proteins, the genes are available and are currently being used as reporters for gene expression studies. Concomttant advances m single photon detection technology have recently made tt possible to measure gene expression nonmvasively m real time. We describe here the apphcatton of bacterial luciferase from Vibrio haweyi and eukaryotic luctferase from Renillu renifomzis as markers for transformation and reporters of gene expression m transgemc plants. The major advantages of the luciferase gene expression system are its simplicity, sensitivity, safety for the investtgator and available nondestructive assay conditions, which permit real-time measurements of gene expression continuously throughout development of transgenic plants.

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Authors and Affiliations

  1. Center for Molecular Biology and Gene Therapy, Loma Linda University, CA

    William H.R Langridge & Aladar A. Szalay

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  1. William H.R Langridge
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  2. Aladar A. Szalay
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Editors and Affiliations

  1. Centro de InvestigaciĂłn y Tecnologia, Instituto Nacional de InvestigaciĂłn y Tecnologia Agraria y Alimentaria, Madrid, Spain

    José M. Martinez-Zapater  & Julio Salinas  & 

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© 1998 Human press Inc, Totowa, NJ

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Langridge, W.H., Szalay, A.A. (1998). Bacterial and Coelenterate Luciferases as Reporter Genes in Plant Cells. In: Martinez-Zapater, J.M., Salinas, J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 82. Humana Press. https://doi.org/10.1385/0-89603-391-0:385

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  • DOI: https://doi.org/10.1385/0-89603-391-0:385

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-391-7

  • Online ISBN: 978-1-59259-268-5

  • eBook Packages: Springer Protocols

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