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Whole-Mount In Situ Hybridization in Plants

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Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 82))

Abstract

Messenger RNA in situ hybridization (ISH) can be defined as a technique for locating specific transcripts. This powerful technique opened the way to study the temporal and spatial patterns of expression of animal and plant genes in a morphological context and on a cell-to-cell basis. Most in situ protocols are based on the hybridtzation of a labeled DNA or RNA probe to tissue sections in which mRNA IS preserved. Radioactive as well as nonradioactive in situ protocols have been applied with success for various tissues originating from plants and animals (1–5).

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Author information

Authors and Affiliations

  1. Laboratorium voor Genetica, Universiteit Gent, Belgium

    Janice de Almeida Engler, Marc Van Montagu & Gilbert Engler

Authors
  1. Janice de Almeida Engler
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  2. Marc Van Montagu
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  3. Gilbert Engler
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Editor information

Editors and Affiliations

  1. Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain

    José M. Martinez-Zapater  & Julio Salinas  & 

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© 1998 Human press Inc, Totowa, NJ

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Engler, J.d.A., Montagu, M.V., Engler, G. (1998). Whole-Mount In Situ Hybridization in Plants. In: Martinez-Zapater, J.M., Salinas, J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 82. Humana Press. https://doi.org/10.1385/0-89603-391-0:373

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  • DOI: https://doi.org/10.1385/0-89603-391-0:373

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-391-7

  • Online ISBN: 978-1-59259-268-5

  • eBook Packages: Springer Protocols

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