Skip to main content
SpringerLink
Log in

Transformation of Arabidopsis thaliana C24 Leaf Discs by Agrobacterium tumefaciens

  • Protocol
Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 82))

Abstract

Transgenic plants have become a very important tool for the study of gene function. The Agrobacterzum binary transformation system (1) allows the precise transfer of a defined piece of DNA (T‐DNA) to the plant genome (2,3) of many plant species. In this way, any desired DNA sequence can be mtroduced into the plant genome. For the small crucifer Arabidopsis thaliana, many transformation protocols are available usmg different tissues as source explant to be transformed with Agrobacterium (3–11).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Hoekema, A., Hirsch, P R, Hooykaas, P. J J, and Schilperoort, R. A. (1983) A binary plant vector strategy based on separation of vn-and T-region of the Agrobacterwm tumefaclens Ti-plasmid. Nature 303, 179–180.

    Article  CAS  Google Scholar 

  2. Hooykaas, P. J. J. and Schtlperoort, R. A. (1992) Agrobacterwm and plant genetic engeneermg Plant Mol. Biol 19, 15–

    CAS  Google Scholar 

  3. Hooykaas, P J. J and BeiJersbergen, A. G M. (1994) The virulence system of Agrobacterlum tumefaclens. Ann Rev Phytopathol 32,157–179.

    Article  CAS  Google Scholar 

  4. Feldmann, K. A. and Marks, M. D (1987) Agrobactenum-mediated transformation of germinating seeds of Arabidopsis thaliana: a none ttssue cultue approach. Mol. Gen. Genet. 208, 1–9.

    Article  CAS  Google Scholar 

  5. Valvekens, D, Van Montagu, M, and Van Lijsebettens, M. (1988) Agrobacterlum tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycm selection. Proc Nat1 Acud Scz USA 85, 5536–5540.

    Article  CAS  Google Scholar 

  6. Van LiJsebettens, M., Vanderhaeghen, R., and Van Montagu, M. (1991) Inserttonal mutagenesis in Arabidopsis thaliana * isolation of a T-DNA-linked mutation that alters leaf morphology Theor Appl Genet 81, 277–284.

    Article  Google Scholar 

  7. Schmidt, R and Willmitzer, L. (1988) High efficiency Agrobacterzum tumefaczens-mediated transformation of Arabrdopsis thaliana leaf and cotyledon explants, Plant Cell Rep. 7, 583–586

    Article  Google Scholar 

  8. Sangwan, R S., Bourgeois, Y, Brown, S., Vasseur, G., and Sangwan-Norreel, B (1992) Characterization of competent cells and early events of Agrobacterzum-mediated genetic transformation of Arabidopsis thaliana Planta 188, 439–456.

    CAS  Google Scholar 

  9. Akama, K., Shnaishi, H, Ohta, S, Nakamura, K., Okada, K, and Shimura, Y. (1992) Efficient transformation of Arabidopsis thaliana. comparison of the efficiencies with various organs, plant ecotypes and Agrobactertum strams. Plant Cell Rep 12,7–11.

    Article  CAS  Google Scholar 

  10. Bechtold, N, Ellis, J., and Pelletier, G (1993) In Planta Agrobacterzum-mediated gene transfer by mfiltration of adult Arabidopsis thaliana plants. C R Acad Sci Paris 316, 1194–1199

    Google Scholar 

  11. Chang, S. S., Park, S K, Krm, B C., Kang, B. J., Krm, D U., and Nam, H. G. (1994) Stable genetic transformation of Arabidopsis thaliana by Agrobactertum maculation m Planta Plant J. 5, 551–558.

    Article  CAS  Google Scholar 

  12. Castle, L. A, Errampalh, D, Atherton, T L, Franzmann, L. H., Yoon, E. S., and Meinke, D. W (1993) Genetic and molecular characterization of embryonic mutants identified following seed transformation in Arabidopsis Mel Gen Genet 241,504–514

    Article  CAS  Google Scholar 

  13. Matzke, A. J. M., Neuhuber, F., Park, Y D., Ambros, P. F, and Matzke, M A (1994) Homology—dependent gene silencing in transgenic plants* epistatic silencing loci contam multtple copies of methylated transgenes. A401 Gen Genet 244,219–229

    CAS  Google Scholar 

  14. Walden, R, Hayashi, H, and Schell, J. (1991) T-DNA as gene tag Plant J 1, 281–288

    Google Scholar 

  15. Koncz, C, Nemeth, K, Redei, G P, and Schell, J (1992) T-DNA msertional mutagenesis in Arabidopsis Plant Mel Biol. 20r 963–976

    Google Scholar 

  16. Walden, R. and Schell, J (1994) Activation T-DNA tagging-a silver bullet approach to isolatmg plant genes. Agro-Food-Industry Hz-Tech 9–12

    Google Scholar 

  17. Walden, R., Fritze, K., Hayashi, H., Mrklashevichs, E., Harlmg, H., and Schell, J (1994) Activation tagging: a means of isolating genes implicated as playing a role in plant growth and development. Plant Mel Btol 26, 1521–1528.

    Article  CAS  Google Scholar 

  18. Van der Graaff, E. and Hooykaas, P J. J (1996) Improvements m the transformanon of Arabidopsis thaliana C24 leaf-discs by Agrobactertum tumefaciens Plant Cell Rep. 15, 572–577.

    Google Scholar 

  19. Pavlica, M., Papes, D, and Nagy, B. (1991) 2&Dichlorophenoxyacetic acid causes chromatm and chromosome abnormalmes in plant cells and mutation in cultured mammalian cells Mutatton Res263,77–81.

    Article  CAS  Google Scholar 

  20. Sangwan, R S., Bourgeois, Y., and Sangwan-Norreel, B. (1991) Genetic transformation of Arabidopsis thaliana zygotic embryos and identificatton of critical parameters influencing transformation. A401 Gen. Genet. 230, 475–485.

    Article  CAS  Google Scholar 

  21. Murashige, T. and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures Plant Physiol 15,473–497

    Article  CAS  Google Scholar 

  22. Gamborg, 0 L, Mtller, R A, and OJtma, K (1968) Nutrients requnements of suspension cultures of soybean root cells Exp Cell Res 50,151–158

    Article  PubMed  CAS  Google Scholar 

  23. Masson, J and Paszkowskr, J (1992) The culture response of Arabidopsis thaliana protoplasts is determined by the growth condmons of donor plants Plant J 2, 829–833

    Article  Google Scholar 

  24. Hood, E E, Gelvm, S B, Melchers, L S, and Hoekema, A (1993) New Agrobacterrum helper plasmrds for gene transfer to plants Truns Res 2,208–218

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Clusius Laboratory, Institute of Molecular Plant AbSciences, Faculty of Mathematics and Natural Sciences, Minneapolis, MN

    Eric van der Graaff & Paul J. J. Hooykaas

Authors
  1. Eric van der Graaff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paul J. J. Hooykaas
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain

    José M. Martinez-Zapater  & Julio Salinas  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Human press Inc, Totowa, NJ

About this protocol

Cite this protocol

van der Graaff, E., Hooykaas, P.J. (1998). Transformation of Arabidopsis thaliana C24 Leaf Discs by Agrobacterium tumefaciens. In: Martinez-Zapater, J.M., Salinas, J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 82. Humana Press. https://doi.org/10.1385/0-89603-391-0:245

Download citation

  • DOI: https://doi.org/10.1385/0-89603-391-0:245

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-391-7

  • Online ISBN: 978-1-59259-268-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation