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Arabidopsis Protocols pp 147–155Cite as

AFLP− Fingerprinting of Arabidopsis

  • Protocol

  • 2003 Accesses

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 82))

Abstract

AFLP− (KeyGene, Wagenmgen, The Netherlands), is a DNA fingerprmtmg technique that visualizes DNA restriction fragments by polymerase chain reaction (PCR) amphfication (1,2). Routinely, 50-100 restriction fragments are amphfied simultaneously and detected on denaturing polyacrylamide gels. The AFLP technique consists of three major steps:

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References

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Author information

Authors and Affiliations

  1. Keygene, Wageningen, The Netherlands

    Pieter Vos

Authors
  1. Pieter Vos
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Editor information

Editors and Affiliations

  1. Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain

    José M. Martinez-Zapater  & Julio Salinas  & 

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© 1998 Human press Inc, Totowa, NJ

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Cite this protocol

Vos, P. (1998). AFLP− Fingerprinting of Arabidopsis. In: Martinez-Zapater, J.M., Salinas, J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 82. Humana Press. https://doi.org/10.1385/0-89603-391-0:147

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  • DOI: https://doi.org/10.1385/0-89603-391-0:147

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-391-7

  • Online ISBN: 978-1-59259-268-5

  • eBook Packages: Springer Protocols

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