Abstract
The identification of cleavage sites for ribozymes within a particular target RNA exclusively on the basis of sequence informatlon often leads to disappointing failures. Even prediction of secondary structures of the target RNA by computer modeling with a more or less exact prediction of single-stranded or loop regions does not guarantee successful cleavage of the target RNA by a synthetic or an expressed ribozyme (1). Expensive screening tests with large numbers of individual ribozymes are the result. As an alternative to this trial-and-error approach, we have recently developed a novel strategy for identification of cleavage sites (2).
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References
Bertrand, E. L., Pictet, R., and Grange, T. (1994) Can hammerhead ribozymes be efficient tools to inactivate gene function? Nucleic Acids Res. 22, 293–300.
Lieber, A. and Strauss, M. (1995) Selection of efficient cleavage sites in target RNAs by using a ribozyme expression library. Mol. Cell Biol. 15, 540–551.
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© 1997 Humana Press Inc.
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Lieber, A., Rohde, M., Strauss, M. (1997). Selection of Efficient Ribozyme Cleavage Sites in Target RNAs. In: Turner, P.C. (eds) Ribozyme Protocols. Methods in Molecular Biology™, vol 74. Humana Press. https://doi.org/10.1385/0-89603-389-9:45
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DOI: https://doi.org/10.1385/0-89603-389-9:45
Publisher Name: Humana Press
Print ISBN: 978-0-89603-389-4
Online ISBN: 978-1-59259-560-0
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