Abstract
In vitro selection methods are powerful tools for the selection of molecules with defined characteristics from complex starting populations (1,2). We have developed a powerful in vitro selection method for analysis of the hairpin ribozyme (3–5). The selection method relies on two sequential RNA-catalyzed reactions, cleavage, and ligation (Fig. 1). In vitro selection of ribozymes, like Darwinian selection of organisms, proceeds through an iterative process consisting of three major steps: mutation, selection of the molecules fulfilling the selection criteria, and replication of the selected molecules.
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© 1997 Humana Press Inc.
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Sargueil, B., Burke, J.M. (1997). In Vitro Selection of Hairpin Ribozymes. In: Turner, P.C. (eds) Ribozyme Protocols. Methods in Molecular Biology™, vol 74. Humana Press. https://doi.org/10.1385/0-89603-389-9:289
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DOI: https://doi.org/10.1385/0-89603-389-9:289
Publisher Name: Humana Press
Print ISBN: 978-0-89603-389-4
Online ISBN: 978-1-59259-560-0
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