Abstract
The catalytic RNA subunit of Escherichia coli RNase P (M1 RNA) is a structure-specific endonuclease that cleaves pre-tRNA substrates in trans. By combining catalytic and substrate RNAs in one RNA molecule, self-cleaving ribozymes have been created (1–3). These constructs were converted to sequence-specific endonucleases by deleting 5′-segments of the tethered tRNA (1) (see Fig. 1). It has also been shown that by linking a guide sequence to the 3′-end of the M1 RNA, a sequence-specific ribozyme could be designed (2) (see Fig. 2).
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References
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© 1997 Humana Press Inc.
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Drainas, D., Krupp, G. (1997). Design and Preparation of Sequence-Specific RNase P Ribozymes. In: Turner, P.C. (eds) Ribozyme Protocols. Methods in Molecular Biology™, vol 74. Humana Press. https://doi.org/10.1385/0-89603-389-9:179
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DOI: https://doi.org/10.1385/0-89603-389-9:179
Publisher Name: Humana Press
Print ISBN: 978-0-89603-389-4
Online ISBN: 978-1-59259-560-0
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