Abstract
Medical applications of enzymes are found in various areas, such as diagnosis, prognosis, and therapy. They are used as soluble or immobilized forms in replacement and detoxification therapies, as scavengers and as antineoplasic drugs, and also for the prevention of clot formation (1). When immobilized enzyme systems are needed for in vivo or ex vivo applications, the use of a biocompatible and a nonthrombogenic immobilization support is an important requirement.
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Fortier, G. (1994) Biomedical applications of enzymes and their poly(ethylene glycol) adducts. Biotechnol. Gen. Engin. Rev. 12, 329–356.
Abuchowski, A., van Est, T., Palczuk, N. C., and Davis, F. F. (1977) Alteration of immunological properties of bovine serum albumin by covalent attachment of polyethylene glycol. J. Biol. Chem. 252, 3578–3581.
Laliberté, M., Gayet, J.-C., and Fortier, G. (1994) Surface modification of horse-radish peroxidase with poly(ethylene glycol) of various molecular masses. II. Relation between the molecular masses of PEG and the stability of horseradish peroxidase-PEG adducts under various denaturing conditions. Biotechnol. Appl. Biochem. 20, 397–413.
D’Urso, E. M. and Fortier, G. (1994) New bioartificial polymeric material: poly(ethylene glycol) crosslinked with albumin. I. Synthesis and swelling properties. J. Bioactive Compat. Polym. 9, 367–387.
D’Urso, E. M. and Fortier, G. (1994) New hydrogel based on poly(ethylene glycol) crosslinked with bovine serum albumin. Biotechnol. Techn. 8, 71–76.
Gayet, J.-C. and Fortier, G. (1996) New bioartificial hydrogels: characterization and physical properties, in Hydrogels and Biodegradable Polymers for Bioapplications, ACS symposium book series # 627 (Ottenbrite, R., Huang, S., and Park, K., eds.), pp. 17–24.
Gayet, J.-C. and Fortier, G. (1996) High water content BSA-PEG hydrogel for controlled release device: evaluation of the drug release properties. J. Control Rel. 38, 177–184.
D’Urso, E. M., Doillon, C., Jean-Francois, J., and Fortier, G. (1995) Polyethylene glycol) crosslinked BSA hydrogels as matrix for enzyme immobilization biomedical applications. Artif. Cells Blood Subst. Immob. Biotechnol. 23, 587–597.
D’Urso, E. M., Jean-Francois, J., and Fortier, G. (1996) New bioartificial hydrogels: biomedical matrix for enzyme immobilization, in Hydrogels and Biodegradable Polymers for Bioapplications, ACS symposium book series # 627 (Ottenbrite, R., Huang, S., and Park, K., eds.), pp. 25–41.
Fortier, G. and Laliberte, M. (1993) Surface modification of horseradish peroxidase with poly(ethylene glycol)s of various molecular masses. Preparation of reagents and characterization of horseradish peroxidase-poly(ethylene glycol) adducts. Biotechnol. Appl. Biochem. 17, 115–130.
Veronese, F. M., Largajolli, R., Boccu, E., Benassi, C. A., and Schiavon, O. (1985) Surface modification of proteins. Activation of monomethoxy-polyethylene glycols by phenylchloroformate and modification of ribonuclease and superoxide dismutase. Appl. Biochem. Biotechnol. 11, 141–152.
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© 1997 Humana Press Inc , Totowa, NJ
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Fortier, G., Demers, N., çois, J.JF., Gayet, JC., D’Urso, E.M. (1997). Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization. In: Bickerstaff, G.F. (eds) Immobilization of Enzymes and Cells. Methods in Biotechnology, vol 1. Humana Press. https://doi.org/10.1385/0-89603-386-4:117
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DOI: https://doi.org/10.1385/0-89603-386-4:117
Publisher Name: Humana Press
Print ISBN: 978-0-89603-386-3
Online ISBN: 978-1-59259-481-8
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