Abstract
The Rhabdoviridae family consists of a large number of nonsegmented negative-strand RNA viruses. As a group, the rhabdoviruses cause many serious plant and animal diseases that have detrimental effects on agricultural productivity, public health, and wildlife populations. The members of the family collectively have an unusually broad host range composed of viruses that infect both plants and animals (1). Many of these viruses are persistently transmitted to their mammalian and plant hosts by insect or arthropod vectors in which they are able to multiply. Consequently, these members of the family may have been able to expand their evolutionary diversity by use of their vectors as intermediate hosts to bridge the boundaries between the animal and plant taxa. Other rhabdoviruses are known to infect fish and aquatic invertebrates, and probably are transmitted via contaminated water.
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References
Wagner, R. R. (1987) Rhabdovirus biology and infection: an overview, in The Rhab-doviruses (Wagner, R. R., ed.), Plenum, New York, pp. 9–74.
Jackson, A. O., Francki, R. I. B., and Zuidema, D. (1987) Biology, structure and replication of plant rhabdoviruses, in The Rhabdoviruses (Wagner, R. R., ed.), Plenum, New York, pp. 427–508.
Wunner, W. H., Calisher, C. H., Dietzgen, R. G., Jackson, A. O., Kitajama, E. W., Lafon, M., Leong, J. C., Nichol, S., Peters, D., Smith, J. S., and Walker, P. J. (1995) Family rhabdoviridae, in Virus Taxonomy: Classification and Nomenclature of Viruses; Sixth Report of the International Committee on Taxonomy of Viruses (Murphy, F. A., Fauquet, C. M., Bishop, D. H. L., Ghabrial, S. A., Jarvis, A. W., Martelli, G. P., Mayo, M. A., and Summers, M. D., eds.), Springer-Verlag, New York, pp. 275–288.
Scholthof, K.-B., Hillman, B. I., Modrell, B., Heaton, L. A., and Jackson, A. O. (1994) Characterization and detection of sc4: a sixth gene encoded by sonchus yellow net virus. Virology 204, 279–288.
Wetzel, T., Dietzgen, R. G., Geering, A. D. W., and Dale, J. L. (1994) Analysis of the nucleocapsid gene of lettuce necrotic yellows rhabdovirus. Virology 202, 1054–1057.
Wetzel, T., Dietzgen, R. G., and Dale, J. L. (1994) Genome organization of lettuce necrotic yellows virus. Virology 200, 401–412.
Francki, R. I. B., Randles, J. W., and Dietzgen, R. G. (1989) Lettuce necrotic yellows virus, in AAB Descriptions of Plant Viruses No. 343.
Jackson, A. O. and Christie, S. R. (1977) Purification and some physicochemical properties of sonchus yellow net virus. Virology 77, 344–355.
Jackson, A. O. and Larkins, B. A. (1976) Influence of ionic strength, pH, and chelation of divalent metals on isolation of polyribosomes from tobacco. Plant Physiol. 51, 5–10.
Wagner, J. D. O., Choi, T.-J., and Jackson, A. O. (1996) Extraction of nuclei from sonchus yellow net rhabdovirus infected plants yields a polymerase that synthesizes viral mRNAs and polyadenylated plus-strand leader RNA. J. Virol. 70, 468–477.
Hunter, B. G., Richardson, J., Dietzgen, R. G., Karu, A., Sylvester, E. S., Jackson, A. O., and Morris, T. J. (1990) Purification and characterization of strawberry crinkle virus. Phytopathology 80, 282–287.
Black, L. M. (1979) Vector monolayers and plant viruses. Adv. Virus Res. 25, 192–271.
Adam, G., Heegard, P., Bog-Hansen, T. C., and Mundry, K. W. (1987) Lectins as probes for the assay of rhabdovirus infections in plants. J. Virol. Methods 17, 263–275.
Francki, R. I. B. (1973) Plant rhabdoviruses. Adv. Virus Res. 18, 257–345.
Jones, R. W. and Jackson, A. O. (1990) Replication of sonchus yellow net virus in infected protoplasts. Virology 179, 815–820.
Milner, J. J. and Jackson, A. O. (1979) Sequence complementarity of sonchus yellow net virus RNA with RNA isolated from the polyribosomes of infected tobacco. Virology 97, 90–99.
Milner, J. J., Hakkaart, M. J. J., and Jackson, A. O. (1979) Subcellular distribution of RNA sequences complementary to sonchus yellow net virus. Virology 98, 497–501.
Gustafson, G. D., Larkins, B. A., and Jackson, A. O. (1981) Comparative analysis of polypeptides synthesized in vivo and in vitro by two strains of barley stripe mosaic virus. Virology 111, 579–587.
Banerjee, A. K. and Barik, S. (1992) Gene expression of vesicular stomatitis virus genome RNA. Virology 188, 417–428.
Wagner, J. D. O. and Jackson, A. O. (1997) Characterization of the components and activity of sonchdus yellow net rhabdovirus polymerase. J. Virol. 71, 2371–2382.
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Jackson, A.O., Wagner, J.D.O. (1998). Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:77
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DOI: https://doi.org/10.1385/0-89603-385-6:77
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