Abstract
Plant reoviruses are classified in three genera in the family Reoviridae: Phytoreovirus, Fijivirus, and Oryzavirus. Fifteen viruses, including possible members, are described (1). With two exceptions, all of them infect plants in gramineae. They possess 10–12 segmented double-stranded RNAs (dsRNAs) as a genome and are transmitted propagatively by leafhoppers or planthoppers. Rice dwarf Phytoreovirus (RDV) is the only plant reovirus whose complete nucleotide sequence is known (2). Rice dwarf virus has 12 genomic segments separated by PAGE. They are numbered from S1 to S12, from the slowest migrating segment, and all the structural and nonstructural proteins of RDV have been assigned (3) (Fig. 1). Genome characterization of other plant reoviruses is reviewed by Uyeda et al. (4).
Genome organization of rice dwarf virus.
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References
Uyeda, I. and Milne, R. G. (1995) Introduction: genome organization, diversity and evolution of plant reoviruses. Semin. Virol. 6, 85–88.
Uyeda, I., Suda, N., Yamada, N., Kudo H., Murao, K., Suga, H., Kimura, I., Shikata, E., Kitagawa, Y., Kusano, T., Sugawara, M., and Suzuki, N. (1994) Nucleotide seqeunce of rice dwarf Phytoreovirus genome segment 2: completion of sequence analyses of rice dwarf virus. Intervirology 37, 6–11.
Suzuki, N. (1995) Molecular analysis of the rice dwarf virus genome. Semin. Virol. 6, 89–95.
Uyeda, I., Kimura I., and Shikata, E. (1995) Characterization of genome structure and establishment of vector cell lines for plant reoviruses. Adv. Virus Res. 45, 249–279.
Murao, K., Suda, N., Uyeda, I., Isogai, M., Suga, H., Yamada, N., Kimura, I., and Shikata, E. (1994) Genomic heterogeneity of rice dwarf phytoreovirus field isolates and nucleotide sequences of genome segment 12. J. Gen. Virol. 75, 1843–1848.
Heinrichs, E. A., Medrano, F. G., and Rapusas, H. R. (1985) Genetic Evaluation for Insect Resistance in Rice, International Rice Research Institute, Los Banos, Philippines.
Redolfi, P. and Boccardo, G. (1974) Fractionation of the doublestranded RNA of maize rough dwarf virus subviral particles. Virology 59, 319–322.
Kawano, S., Shikata E., and Senboku, T. (1983) Purification and morphology of rice ragged stunt virus. J. Fac. Agr. Hokkaido Univ. 61, 209–218.
Omura, T., Minobe, Y., Kimura, I., Hibino, H., Tsuchizaki, T., and Saito, Y. (1983) Improved purification procedure and RNA segments of rice ragged stunt virus. Ann. Phytopathol. Soc. Japan 49, 670–675.
Morris, T. J., Dodds, J. A., Hillman, B., Jordan, R. L., Lommel, S. A., and Tamaki, S. J. (1983) Viral specific dsRNA: diagnostic value for plant virus disease identification. Plant Mol. Biol. Rep. 1, 27–30.
Dlieu, P. and Bar-Joseph, M. (1989) Rapid isolation of double stranded RNA segments from disulphide crosslinked polyacrylamide gels. J. Virol. Methods 24, 77–84.
Diaz-Ruiz, J. R. and Kaper, J. N. (1978) Isolation of viral doublestranded RNAs using LiCl fractionation procedure. Prep. Biochem. 8, 117.
Suga, H., Uyeda, I., Yan, J., Murao, K., Kimura, I., Tiongco, E. R., Cabauatan, P., and Kcganezawa, H. (1995) Heterogeneity of rice ragged stunt oryzavirus genome segment 9 and its segregation by insect vector transmission. Arch. Virol. 140, 1503–1509.
Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium lthiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.
Dodds, J. A., Morris, T. J., and Jordan, R. L. (1984) Plant viral doublestranded RNA. Ann. Rev. Phytopathol. 22, 151–168.
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Uyeda, I., Lee, BC., Ando, Y., Suga, H., He, YK., Isogai, M. (1998). Reovirus Isolation and RNA Extraction. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:65
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DOI: https://doi.org/10.1385/0-89603-385-6:65
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