Abstract
Geminiviruses, named for their unique geminate capsid morphology, have small single-stranded (ss) circular DNA genomes that replicate in the nuclei of infected cells via a double stranded (ds) DNA intermediate. They are responsible for economically devastating diseases in a wide variety of crop species from cereals to legumes; it is thus important to gain a better understanding of their epidemiology, genetic diversity, and molecular mechanisms of replication and pathogenicity, for the design of effective resistance strategies (for reviews, see refs. 1 and 2). Each geminate particle encapsidates a circular single stranded genomic component of between 2.5 and 3 kb. Viruses in the taxonomic family Geminiviridae are classified into three genera (Mastrevirus, Curtovirus, Begomovirus), based on their host range, genome organization, and vector species (3,4). Mastreviruses, such as maize streak virus (MSV) and wheat dwarf virus (WDV), have monopartite genomes, are transmitted by leaf-hopper species, and, with a few exceptions, infect monocotyledonous plants. Begomoviruses, such as bean and tomato golden mosaic viruses (BGMV and TGMV), are transmitted by whiteflies (Bemisia tabacci) and all infect dicotyledonous plants; most have bipartite genomes, although there are some viruses in this group that apparently have monopartite genomes. Curtoviruses, such as beet curly top and tomato pseudo curly top viruses (BCTV and TPCTV), occupy an intermediate position between Mastreviruses and Begomoviruses, in that these viruses have monopartite genomes and are transmitted by leafhopper species, but only infect dicotyledonous hosts.
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References
Lazarowitz, S. G. (1992) Geminiviruses: genome structure and gene function. Crit. Rev. Plant Sci. 11, 327–349.
Timmermans, M. C. P., Das, O. P., and Messing, J. (1994) Geminiviruses and their use as extrachromosomal replicons. Annu. Rev. Plant Physiol. Plant Mol. Biol. 45, 79–112.
Briddon, R. W. and Markham, P. G. (1995) Geminiviridae, in Virus Taxonomy: Sixth Report of the International Committee on the Taxonomy of Viruses (Murphy, F. A., Fauquet, C. M., Bishop, D. H. L., Ghabrial, S. A., Jarvis, A. W., Martelli, G. P., Mayo, M. A., and Summers, M. D., eds.), Springer-Verlag, Vienna, pp. 158–165.
Rybicki, E. P. (1994) A phylogenetic and evolutionary justification for three genera of Geminiviridae. Arch. Virol. 139, 49–77.
Bisaro, D. M. (1996) Geminivirus replication, in Eukaryotic DNA Replication (De Pamphilis, M., ed.), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Laufs, J., Traut, W., Heyraud, F., Matzeit, V., Rogers, S. G., Schell, J., and Gronenborn, B. (1995) In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus. Proc. Natl. Acad. Sci. USA 92, 3879–3883.
Laufs, J., Jupin, I., David, C., Schumacher, S., Heyraud-Nitschke, F., and Gronenborn, B. (1995) Geminivirus replication: genetic and biochemical characterisation of Rep protein function, a review. Biochimie 77, 765–773.
Stanley, J. (1995) Analysis of African cassava mosaic virus recombinants suggests strand nicking occurs within the conserved nonanucleotide motif during the initiation of rolling circle replication. Virology 206, 707–712.
Orozco, B. M. and Hanley-Bowdoin, L. (1996) A DNA structure is required for geminivirus replication origin function. J. Virol. 70, 148–158.
von Wechmar, M. B. and Milne, R. G. (1983) Purification and serology of a South African isolate of maize streak virus, in Procedings International Maize Virus Disease Colloquium and Workshop, 2–6 August 1992 (Gordon, D. T., Knoke, J. K., Nault, L. R., and Ritter, R. M., eds.), Ohio State University, Wooster, OH.
Hamilton, W. D. O., Saunders, R. C., Coutts, R. H. A., and Buck, K. W. (1981) Characterisation of tomato golden mosaic virus as a geminivirus. FEMS Microbiol. Lett. 11, 263–267.
Czosnek, H., Ber, R., Antignus, Y., Cohen, S., Navot, N., and Zamir, D. (1988) Isolation of tomato yellow leaf curl virus, a geminivirus. Phytopathology 78, 508–512.
Sequeira, J. C. and Harrison, B. D. (1982) Serological studies on cassava latent virus. Ann. Appl. Biol. 101, 33–42.
Mullineaux, P. M., Donson, J., Morris-Krsinich, B. A. M., Boulton, M. I., and Davies, J. W. (1984) The nucleotide sequence of maize sreak virus DNA. EMBO J. 3, 3063–3068.
Donson, J., Morris-Krsinisch, B. A. M., Mullineaux, P. M., Boulton, M. I., and Davies, J. W. (1984) A putative primer for second-strand synthesis of maize streak virus is virion-associated. EMBO J. 3, 3069–3073.
Howell, S. H. (1984) Physical structure and genetic organisation of the genome of maize streak virus (Kenyan isolate). Nucleic Acids Res. 12, 7359–7375.
Stanley, J. and Townsend, R. (1985) Characterisation of DNA forms associated with cassava latent virus infection. Nucleic Acids Res. 13, 2189–2206.
Hirt, B. (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Biol. 26, 365–369.
Xie, Q., Suérez-López, P., and Gutiérrez, C. (1995) Identification and analysis of a retinoblastoma binding motif in the replication protein of a plant DNA virus: requirement for efficient viral DNA replication. EMBO J. 14, 4073–4082.
Gilbertson, R. L., Faria, J. C., Hanson, S. F., Morlaes, F. J., Ahlquist, P., Maxwell, D. P., and Russel, D. R. (1991) Cloning of the complete genomes of four bean-infecting geminiviruses and determining their infectivity by electric discharge particle acceleration. Phytopathology 81, 980–985.
Sunter, G., Coutts, R. H. A., and Buck, K. W. (1984) Negatively supercoiled DNA from plants infected with a single stranded DNA virus. Biochem. Biophys. Res. Commun. 118, 747–752.
Srivistava, K. M., Hallan, V., Raizada, R. K., Chandra, G., Singh, B. P., and Sane, P. V. (1995) Molecular cloning of Indian tomato leaf curl virus following a simple method of concentrating the supercoiled replicative form of viral DNA. J. Virol. Methods 51, 297–304.
Bendahmane, M., Schalk, H.-J., and Gronenborn, B. (1995) Identification and characterisation of wheat dwarf virus from France using a rapid method for geminivirus DNA preparation. Phytopathology 85, 1449–1455.
Ish-Horowitz, D. and Burke, J. F. (1982) Rapid and efficient cosmid vector cloning. Nucleic Acids Res. 9, 2989–2998.
Rybicki, E. P. and Hughes, F. L. (1990) Detection and typing of maize streak virus and other distantly related viruses of grasses by polymerase chain reaction amplification of a conserved viral sequence. J. Gen. Virol. 71, 2519–2526.
Rojas, M. R., Gilbertson, R. L., Russel, D. R., and Maxwell, D. P. (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease 77, 340–347.
Briddon, R. W., Prescott, A. G., Luness, P., Chamberlin, L. C. L., and Markham, P. G. (1993) Rapid production of full-length, infectious geminivirus clones by abutting primer PCR (AbP-PCR). J. Virol. Methods 43, 7–20.
Patel, V. P., Rojas, M. R., Paplomatas, E. J., and Gilbertson, R. L. (1993) Cloning biologically active geminivirus DNA using PCR and partially overlapping primers. Nucleic Acids Res. 21, 1325–1326.
Tan, P. H. N., Wong, S. M., Wu, M., Bedford, I. D., Saunders, K., and Stanley, J. (1995) Genome organisation of ageratum yellow vein virus, a monopartite white-fly-transmitted geminivirus isolated from a common weed. J. Gen. Virol. 76, 2915–2922.
Miller, G. L. and Golder, R. H. (1950) Buffers of pH 2 to 12 for use in electrophoresis. Arch. Biochem. 29, 420–423.
Sambrook, J. Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
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Palmer, K.E., Schnippenkoetter, W.H., Rybicki, E.P. (1998). Geminivirus Isolation and DNA Extraction. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:41
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DOI: https://doi.org/10.1385/0-89603-385-6:41
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