Abstract
Oryza sativa L. has three subspecies: indica, japonica, and javanica. Subsp. indica grows well in southern temperate and tropical regions; subsp. japonica grows well in cooler climates such as Japan; and subsp. javanica is grown mostly in the Americas and Europe. Several viruses infect rice, causing devastating losses in yield. Tungro disease is caused by an association of an RNA genome virus (rice tungro spherical virus) and a DNA genome virus (rice tungro bacilliform virus). Estimated annual yield losses caused by Tungro virus infection of rice exceed $1.5 billion (1). Rice ragged stunt virus causes the second most important viral disease, with economic losses exceeding $140 million annually. Several tenuiviruses are also major pathogens in various rice-growing regions. These include rice grassy stunt virus, which is prevalent in the Philippines; rice stripe virus, which is often found in Japan; and rice hoja blanca virus, which is endemic in Latin America and occurs in sporadic but disastrous outbreaks. Novel biotechnological approaches for resistance, using various pathogen-derived genes, are being explored (2). The production and thorough molecular analysis of transgenic plants by methods such as those described in this chapter and in Chapter 41 are vital toward evaluation of the efficacy of these new approaches.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Herdt, R. W. (1991) Research priorities for rice biotechnology, in Rice Biotechnology (Khush, G. S. and Toenniessen, G. H., eds.), Alden, Oxford, pp. 19–54.
Huntley, C. C. and Hall, T. C. (1996) Interference with brome mosaic virus replication in transgenic rice. Mol. Plant-Microbe Interact. 9, 164–170.
Sanford, J. C., Klein, T. M., Wolf, E. D., and Allen, N. (1987) Delivery of substances into cells and tissues using a particle bombardment process. Part. Sci. Technol. 5, 27–37.
Sanford, J. C., Smith, F. D., and Russell, J. A. (1992) Optimizing the biolistic process for different biological applications, in Recombinant DNA, Part H, vol. 217 (Wu, R., ed.), Academic, San Diego, CA, pp. 483–509.
Heiser, W. (1992) Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System. Bio-Rad, Hercules, CA.
Hoshikawa, K. (1989) The growing rice plant: an anatomical monograph. Nobunkyo, Tokyo.
Li, L., Qu, R., de Kochko, A., Fauquet, C., and Beachy, R. N. (1993) An improved rice transformation system using the biolistic method. Plant Cell Rep. 12, 250–255.
Shimamoto, K., Terada, R. T., Izawa, T., and Fujimoto, H. (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 238, 274–276.
Toryama, K., Arimoto, Y., Uchimiya, H., and Hinata, K. (1988) Transgenic rice plants after direct gene transfer into protoplasts. Biotechnology 6, 1072–1074.
Hayashimoto, A., Zhijian, L., and Murai, N. (1990) A polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants. Plant Physiol. 93, 857–863.
Battraw, M. and Hall, T. C. (1992) Expression of a chimeric neomycin phosphotransferase II gene in first and second generation transgenic rice plants. Plant Sci. 86, 191–202.
Hiei, Y., Ohta, S., Komari, T., and Kumashiro, T. (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacteriumand sequence analysis of the boundaries of the T-DNA. Plant J. 6, 271–282.
Dong, J. J., Teng, W. M., Buchholz, W. G., and Hall, T. C. (1996) Agrobacterium-mediated transformation of Javanica rice. Mol. Breeding 2, 267–276.
Linsmaier, E. and Skoog, F. (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127.
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
Dekeyser, R., Claes, B., Marichal, M., Van Montagu, M., and Caplan, A. (1989) Evaluation of selectable markers for rice transformation. Plant Physiol. 90, 217–223.
Mikkelson, D. (1987) University of California, Davis, personal communication.
Battraw, M. J. and Hall, T. C. (1990) Histochemical analysis of CaMV 35S promoter-β-glucuronidase gene expression in transgenic rice plants. Plant Mol. Biol. 15, 527–538.
Flavell, R. B. (1994) Inactivation of gene expression in plants as a consequence of specific sequence duplication. Proc. Natl. Acad. Sci. USA 91, 3490–3496.
Finnegan, J. and McElroy (1994) Transgene inactivation: plants fight back! Biotechnology 12, 883–888.
Matzke, M. A. and Matzke, A. J. M. (1995) How and why do plants inactivate homologous (trans)genes? Plant Physiol. 107, 679–685.
Tomes, D. (1994) Pretreatment of microprojectiles prior to using in a particle gun. Pioneer Hi-Bred International, International Patent no. W094/17195.
Kumpatla, S. P. (1997) Transgene integrity, chimerism, silencing and stability in rice. Ph.D. Thesis, Texas A&M University.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc.
About this protocol
Cite this protocol
Buchholz, W.G., Teng, W., Wallace, D., Ambler, J.R., Hall, T.C. (1998). Production of Transgenic Rice (Oryza sativa subspecies japonica cv. Taipei 309). In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:383
Download citation
DOI: https://doi.org/10.1385/0-89603-385-6:383
Publisher Name: Humana Press
Print ISBN: 978-0-89603-385-6
Online ISBN: 978-1-59259-566-2
eBook Packages: Springer Protocols