Abstract
Genetic transformation of plants is a technique by which foreign DNA is introduced into plant cells, leading to regeneration of transgenic plants, with new features resulting from integration and expression of the foreign DNA. This technique has become an indispensable tool, both for plant biotechnologists to incorporate agronomically useful genes into crops, and for plant molecular biologists to test a particular structural gene or promoter in plant cells. Plant virologists use this technique to study the relationship between a viral pathogen and its host, and to introduce antiviral genes into plants to combat viral diseases.
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References
Vasil, V., Castillo, A. M., Fromm, M. E., and Vasil, I. K. (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. BioTechnology 10, 667–647.
Vasil, V., Srivastava, V., Castillo, A. M., Fromm, M. E., and Vasil, I. K. (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. BioTechnology 11, 1553–1558.
Weeks, J. T., Anderson, O. D., and Blechl, A. E. (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol. 102, 1077–1084.
Becker, D., Brettschneider, R., and Lörz, H. (1994) Fertile transgenic wheat from microprojectile bombardment of scutella tissue. Plant J. 5, 299–307.
Nehra, N. S., Chibbar, R. N., Leung, N., Caswell, K., Mallard, C., Steinhauer, L., Baga, M., and Kartha, K. K. (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J. 5, 285–297.
Klein, T. M., Wolf, E. D., Wu, R., and Sanford, J. C. (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 237, 70–73.
Jefferson, R. A. (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5, 387–405.
Spencer, T. M., Gordon-Kamm, W., Daines, R. J., Start, W. G., and Lemaux, P. G. (1990) Bialaphos selection of stable transformants from maize cell culture. Theor. Appl. Genet. 79, 625–631.
Goff, S. A., Klein, T. M., Roth, B. A., Fromm, M. E., Cone, K. C., Radicella, J. P., and Chandler, V. L. (1990) Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. EMBO J. 9, 2517–2522.
Ludwig, S., Bowen, B., Beach, L., and Wessler, S. (1990) A regulatory gene as a novel visible marker for maize transformation. Science 247, 449,450.
McCormac, A. C., Wu, H., Bao, M., Wang, Y.-B., Xu, R., Elliott, M. C., and Chen, D. F. (1997) The use of visual markers as cell-specific reporters of Argobacterium-mediated T-DNA delivery to wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.). Euphytica, in press.
Chen, D. F., Dale, P. J., Heslop-Harrison, J. S., Snape, J. W., Harwood, W., Bean, S., and Mullineaux, P. M. (1994) Stability oftransgenes and presence of N6 methyladenine DNA in transformed wheat cells. Plant J. 5, 429–436.
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© 1998 Humana Press Inc.
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Chen, DF. (1998). Genetic Transformation of Wheat. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:373
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DOI: https://doi.org/10.1385/0-89603-385-6:373
Publisher Name: Humana Press
Print ISBN: 978-0-89603-385-6
Online ISBN: 978-1-59259-566-2
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