Skip to main content

Transformation of Tomato

  • Protocol
Plant Virology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 81))

Abstract

Since 1986, when Beachy and coworkers (1) first published a protection of transgenic tobacco plants expressing the tobacco mosaic virus (TMV) coat protein (CP) against TMV infection, transgenic plants have become an important tool in plant virology. Coat protein transgenic plants have been used successfully to obtain protection against many different plant viruses (2). In addition, transgenic plants expressing viral gene products can be used to study the function of one particular gene in the viral life cycle or in the interaction with specific host plants.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Powell Abel, P. A., Nelson, R. S., De, B., Hoffmann, N., Rogers, S. G., Fraley, R. T, and Beachy, R. N. (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232, 738–743.

    Google Scholar 

  2. Beachy, R. N., Loesch-Fries, S., and Tumer, N. E. (1990) Coat protein mediated resistance against virus infection. Ann. Rev. Phytopathol. 28, 451–474.

    Article  CAS  Google Scholar 

  3. Filatti, J. J., Kiser, J., Rose, B., and Comai, L. (1987) Efficient transformation of tomato and the introduction and expression of a gene for herbicide tolerance, in Tomato Biotechnology (Nevins, D. J. and Jones, R. A., eds.), Liss, New York, pp. 199–210.

    Google Scholar 

  4. Smith, C. J. S., Watson, C. F., Ray, J., Bird, C. R., Morris, P. C., Schuch, W., and Grierson, D. (1988) Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334, 724–726.

    Article  CAS  Google Scholar 

  5. Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 12, 8711–8721.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc.

About this protocol

Cite this protocol

Pfitzner, A.J.P. (1998). Transformation of Tomato. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:359

Download citation

  • DOI: https://doi.org/10.1385/0-89603-385-6:359

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-385-6

  • Online ISBN: 978-1-59259-566-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics