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Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation

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Plant Virology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 81))

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Abstract

Transgenic plants expressing the coat protein (CP), and/or the RNA transcript of the CP gene of many RNA viruses, may be protected against the virus from which the CP gene was derived, and, in some cases, against related viruses (for further details, see Chapter 3). Since it is not possible to predict from the literature when protein expression is not required, the procedure described in this chapter will be designed to obtain CP expression. The initiation codon of the CP may be deleted or mutated to prevent production of the protein.

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© 1998 Humana Press Inc.

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Wallis, C.V., Boulton, M.I. (1998). Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:341

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  • DOI: https://doi.org/10.1385/0-89603-385-6:341

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-385-6

  • Online ISBN: 978-1-59259-566-2

  • eBook Packages: Springer Protocols

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