Abstract
At least 20 tymovirus species are known. The generic acronym, tymovirus, comes from turnip yellow mosaic virus (TYMV), the name of the first to be described (1), now the type species. The tymoviruses infect dicotyledonous angiosperms, mostly those that have the C3 photosynthetic pathway; few have been recorded in crop plants, and none are known to infect monocotyledonous angiosperms. They cause bright yellow mosaics, vein-clearing, and mottling. Their natural vectors, when known, are beetles, mostly halticid or galerucid flea beetles. Only four tymoviruses have been recorded as seed-borne (2).
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References
Markham, R. and Smith, K. M. (1949) Studies on the virus of turnip yellow mosaic. Parasitology 39, 330–342.
Brunt, A., Crabtree, K., Dallwitz, M., Gibbs, A., and Watson, L. (1996) Viruses of Plants. CAB International, Wallingford, UK.
Matthews, R. E. F. (1991) Plant Virology, 3rd ed. Academic, New York, pp. 231–239; 404–406.
Rozanov, M. N., Koonin, E. V., and Gorbalenya, A. E. (1992) Conservation of the putative methyltransferase domain: a hallmark of the “Sindbis-like” supergroup of positive-strand RNA viruses. J. Gen. Virol. 73, 2129–2134.
Bransom, K. L. and Dreher, T. W. (1994) Identification of the essential cysteine and histidine residues of the turnip yellow mosaic virus protease. Virology 198, 148–154.
Rozanov, M. N., Drugeon, G., and Haenni, A.-L. (1995) Papain-like proteinase of turnip yellow mosaic virus: a prototype of a new viral proteinase group. Arch. Virol. 140, 273–288.
Gorbalenya, A. E. and Koonin, E. V. (1989) Viral proteins containing the purine NTP-binding sequence pattern. Nucleic Acid Res. 17, 8413–8440.
Kamer, G. and Argos, P. (1984) Primary structural comparion of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acid Res. 12, 7269–7282.
Kadaré, G., Rozanov, M., and Haenni, A.-L. (1995) Expression of the turnip yellow mosaic virus proteinase in Escherichia coli and determination of the cleavage site within the 206 kDa protein. J. Gen. Virol. 76, 2853–2857.
Ding, S., Howe, J., Keese, P., Mackenzie, A., Meek, D., Osorio-Keese, M., Skotnicki, M., Srifah, P., Torronen, M., and Gibbs, A. (1990) The tymobox, a sequence shared by most tymoviruses: its use in molecular studies of tymoviruses. Nucleic Acid Res. 18, 1181–1187.
Matthews, R. E. F. (1960) Properties of nucleoprotein fractions isolated from turnip yellow mosaic virus preparations. Virology 12, 521–539.
Steere, R. L. (1956) Purification and properties of tobacco ringspot virus. Phytopathology 46, 60–69.
Canady, M. A., Day, J., and McPherson, A. (1995) Preliminary X-ray diffraction analysis of crystals of turnip yellow mosaic virus (TYMV). Proteins 21, 78–81.
Both, G. W. and Air, G. M. (1979) Nucleotide sequence for the N-terminal region of the matrix protein of influenza virus. Eur. J. Biochem. 96, 363–372.
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© 1998 Humana Press Inc.
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Gibbs, A., Mackenzie, A.M. (1998). Tymovirus Isolation and Genomic RNA Extraction. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:219
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DOI: https://doi.org/10.1385/0-89603-385-6:219
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