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Furovirus Isolation and RNA Extraction

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Plant Virology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 81))

Abstract

The Furovirus (fungus-transmitted rod-shaped) group, whose type member is soil-borne wheat mosaic virus (SBWMV) (1), is a very heterogeneous group infecting a wide variety of hosts. The group consists of viruses that are naturally transmitted by soil-borne fungal vectors, generally of the family Plasmodiophorales. Virions are rigid rods and contain two or more genome components consisting of positive-sense RNA. Furoviruses typically infect roots, but can also invade, or be inoculated to, leaves. As sequence data for different furoviruses has become available, it has become clear that there are significant differences in genetic organization within the group (2,10). We find it convenient to divide the viruses into two major subgroups (Fig. 1), which are distinguished by the nature of the protein(s) putatively involved in cell-to-cell movement. Subgroup 1 consists of SBWMV, which appears to have a movement protein (MP) of the type characterized by the 30-kDa protein of tobacco mosaic virus (TMV) (6). Subgroup 2 furoviruses (beet necrotic yellow vein virus [BNYVV], peanut clump virus [PCV], and potato moptop virus [PMTV]) do not have a TMV type MP but instead possess a cluster of three slightly overlapping genes known as the triple gene block (TGB), which has been shown for BNYVV (11), and is presumed for the other viruses (7–9) to mediate viral cell-to-cell movement.

Genetic organization of characterized members of the furovirus group. RNA1 of PMTV has not yet been sequenced, but presumably encodes the viral replicase. ORFs are represented as hollow rectangles. Asterisks indicate suppressible termination codons. TGB, tripe gene block; Cys-rich, cysteine rich protein; C P, coat protein; mvt, putative movement protein of SBWMV; AA, poly(A) tail; black rectangle, consensus core polymerase motif (12).

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Authors and Affiliations

  1. CNRS (IBMP), Strasbourg, France

    Salah E. Bouzoubaa

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  1. Salah E. Bouzoubaa
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Editor information

Editors and Affiliations

  1. Plant Virology Group School of Biological Sciences, University of Bristol, UK

    Gary D. Foster

  2. Department of Botany, University of Leicester, UK

    Gary D. Foster  & Sally C. Taylor  & 

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© 1998 Humana Press Inc.

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Bouzoubaa, S.E. (1998). Furovirus Isolation and RNA Extraction. In: Foster, G.D., Taylor, S.C. (eds) Plant Virology Protocols. Methods in Molecular Biology™, vol 81. Humana Press. https://doi.org/10.1385/0-89603-385-6:107

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  • DOI: https://doi.org/10.1385/0-89603-385-6:107

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-385-6

  • Online ISBN: 978-1-59259-566-2

  • eBook Packages: Springer Protocols

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