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Southern Analysis of Transgenic Tobacco Plants

  • Dawn Worrall
Part of the Methods in Molecular Biology™ book series (MIMB, volume 81)

Abstract

Southern analysis (1) is routinely carried out to determine whether a plant regenerated from tissue-culture has been transformed with foreign DNA. The technique of Southern analysis begins with the extraction of genomic DNA from the plant, digestion of the DNA with diagnostic-restriction enzymes, and fractionation of the restricted DNA by agarose gel electrophoresis. Following the transfer of the fractionated DNA to a nylon membrane by capillary blotting (Southern blotting), a radioactively labeled fragment of the foreign DNA is used for the detection of homologous sequences within the plant genomic DNA. This technique allows not only the detection of foreign DNA, but also an estimation of the number of copies and the arrangement of the foreign gene(s) within the plant genome.

Keywords

Southern Analysis Isoamyl Alcohol Geiger Counter Ethidium Bromide Solution Saran Wrap 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Dawn Worrall
    • 1
  1. 1.The Plant LaboratoryUniversity of YorkUK

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