Abstract
Until recently, the culture of Helicobacter pylori in vitro has only been achievable with complex undefined media supplemented with blood products, such as Brain-heart infusion (BHI) medium, blood agar, chocolate agar, or lysed blood agar (1). Substrate utilization and metabolic activity during growth on these media is difficult to define; this has led to the use of techniques such as nuclear magnetic resonance (NMR) spectroscopy to investigate the metabolic pathways active in H. pylori (e.g., refs. 2–5). Defined media for the growth of H. pylori have now been described (6,7), however, and may facilitate future metabolic studies in this organism by more conventional techniques.
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References
Goodwin, C. S and Armstrong, J. A (1990) Microbiological aspects of Helicobacter pylori. Eur. J, clin Microbiol. Infect Dis. 9(1), 1–13.
Mendz, G L and Hazell, S. L (1993) Glucose phosphorylation in Helicoacyer. pylori Archives Bzochem. Bzophys 300(1), 522–525.
Mendz, G. L., Hazell, S L, and Burns, B. P. (1993) Glucose utilisation and lactate production by Helicobacter pylori. J. tGen. Microbiol 139, 3023–3028.
Chalk, P. A., Roberts, A D, and Blows, W. M (1994) Metabolism of pyruvate and glucose by intact cells of Helicobacter pylori studied by 13C NMR spectros copy. Microbioiogy 140, 2085–2092.
Mendz, G. L, Hazell, S. L., and Burns, P. (1994) The Entner-Doudoroff pathway in Helicobacter pylori. Arch Biochem. Biophys. 312, 349–356.
Reynolds, D. J. and Penn, C W. (1994) Characteristics of Helicobacter pylori growth in a defined medium and determination of its amino acid requirements Microbiology 140, 2649–2656.
Nedenskov, P (1994) Nutritional requirements for growth of Helicobacter pylori. Appl Environ Microbiol 60(9), 3450–3453.
Williams, H. J., Gao, Y., and Scott, I. (1991) Simple devices for sterile aeration and mixing in NMR cell metabolism studies. J Magn Reson. 94, 405–407.
Lundberg, P., Harmsen, E., Ho, C., and Vogel, H. J. (1990) Nuclear magnetic resonance studies of cellular metabolism. Anal Biochem 191, 193–222.
Jeffrey, F. M. H., Rajagopal, A., Malloy, C. R., and Sherry, A. D. (1991) 13C-NMR. a simple yet comprehensive method for analysis of intermediary metabolism. TIBS 16, 5–10
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© 1997 Humana Press Inc., Totowa, NJ
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Chalk, P.A., Roberts, A.D., Davison, A.A., Kelly, D.J., White, P.J. (1997). Use of NMR to Study H. pylori Metabolism. In: Clayton, C.L., Mobley, H.L.T. (eds) Helicobacter pylori Protocols. Methods in Molecular Medicine, vol 8. Humana Press. https://doi.org/10.1385/0-89603-381-3:69
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DOI: https://doi.org/10.1385/0-89603-381-3:69
Publisher Name: Humana Press
Print ISBN: 978-0-89603-381-8
Online ISBN: 978-1-59259-592-1
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