Abstract
A number of methods are currently available for the detection of Helicobacter pylori, including serology, culture, histology, and isotope breath tests. All have relative advantages and disadvantages of sensitivity, specificity, convenience, expense, and immediacy. The polymerase chain reaction (PCR) was an obvious choice of test once the technique became available to many laboratories. PCR tests for various bacteria, including H. pylori, are available on a wide range of specimens, including cultures and direct clinical samples. The ideal PCR test for H. pylori would be highly sensitive and specific, cheap if performed in bulk, and could produce results much faster than would be possible with culture.
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References
Clayton, C., Kleanthous, K., and Tabaqchali, S. (1991) Detection and identitication of Helicobacter pylori by the polymerase chain reaction. J Clin Pathol. 44, 515–516.
Owen, R. J., Hurtado, A., Banatvala, N., Abdi, Y., Davies, G. R, Feldman, R., and Hardie, J. M. (1994) Conservation of the cytotoxin-associated (cagA) gent of Helicobacter pylori and investigation of association with vacuolating-cytotoxin activity and gastroduodenal disease. Fems Immunol Med Microbiol 9, 307–315.
Ho, S. A., Hoyle, J. A., Lewis, F. A., Seeker, A. D., Cross, D., Mapstone, N. P., Dixon, M. F., Wyatt, J. I., Tompkins, D. S., Taylor, G. R., and Quirke, P. (1991) Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals. J. Clin. Microbiol 29, 2543–2549.
Bemelman, W. A., Bosma, A., Wiersma, P. H, Rauws, E. A., and Brummelkamp, W. H. (1993) Role of Helicobacter pylori in the pathogenesis of complications of Meckel’s diverticula. Eur. J. Surg. 159, 171–175.
Kestemberg, A., Marino, G., De Lima, E., Garcia, F. T., Carrascal, E., and Arredondo, J. L. (1993) Gastric heterotopic mucosa in the rectum with Helicobacter pylori-like organisms: a rare cause of rectal bleeding. Int J Colorectal Disease 8, 9–12.
Thomas, J. E., Gibson, G. R., Darboe, M. K., Dale, A., and Weaver, L. T. (1992) Isolation of Helicobacter pylori from human faeces. Lancet 340, 1194,1195.
Kelly, S M., Pitcher, M. C., Farmery, S. M., and Gibson, G. R. (1994) Isolation of Helicobacter pylori from feces of patients with dyspepsia in the United Kingdom. Gastroenterology 107, 1671–1674.
Mapstone, N. P., Lynch, D. A., Lewis, F. A., Axon, A. T., Tompkins, D. S, Dixon, M. F., and Quirke, P. (1993) PCR identification of Helicobacter pylori in faeces from gastritis patients. Lancet 341, 447.
van Zwet, A., Thijs, J. C., Kooistra, S. A., Schirm, J, and Snijder, J A (1994) Use of PCR with feces for detection of Helicobacter pylori infections in patients. J Clin Pathol 32, 1346–1348.
Mapstone, N. P., Lynch, D. A., Lewis, F. A., Axon, A. T., Tompkins, D. S., Dixon, M. F., and Quirke, P (1993) Identification of Helicobacter pylori DNA in the mouths and stomachs of patients with gastritis using PCR. J. Clin Pathol 46, 540–543.
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© 1997 Humana Press Inc., Totowa, NJ
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Mapstone, N.P. (1997). The Detection of H. pylori by the Polymerase Chain Reaction. In: Clayton, C.L., Mobley, H.L.T. (eds) Helicobacter pylori Protocols. Methods in Molecular Medicine, vol 8. Humana Press. https://doi.org/10.1385/0-89603-381-3:31
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DOI: https://doi.org/10.1385/0-89603-381-3:31
Publisher Name: Humana Press
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