Abstract
Transposable elements are well-known genetic tools that enable the geneticist to generate mutations by disrupting the linear continuity of a specific gene and, consequently, affect its expression. This approach, in addition to providing an efficient way to create mutants, which are easily detectable because of the acquisition of drug resistance phenotype (when a drug-resistance transposable element is used), also provides an easy way to recover and identify the mutated gene. Several genetic systems have been developed in the past for many bacterial species and have permitted random and generalized mutagenesis to be performed on given genomes by introducing, via conjugation, suicide plasmids harboring transposable elements. Unfortunately, such transposon delivery systems have not yet been found to be functional in Helicobacter pylori.
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Seifert, H S., So, M., and Heffron, F. (1985) Shuttle mutagenesis. a method of transposon mutagenesis for Saccharomyces cerevisiae. Proc Natl. Acad. Sci USA 83, 735–739.
Labigne-Roussel, A., Courcoux, P., and Tompkins, L (1988) Gene disruption and replacement as a feasible approach for mutagenesis of Campylobacter jejuni. J Bacteriol 170, 1704–1708
Ferrero, R. L., Cussac, V., Courcoux, P., and Labigne, A. (1992) Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange J Bacteriol. 174, 4212–4217.
Labigne, A., Courcoux, P., and Tompkins, L. (1992) Cloning of Cumpylobacter jejuni genes required for leucine biosynthesis, and construction of leu-negative mutant of C. jejuni by shuttle mutagenesis. Res Microbiol 143, 15–26.
Boyer, H. W. and Roulland-Dussoix, D. (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol. Biol 41, 459–472.
Bachmann, B. J. (1996) Derivations and genotypes of some mutant derivatives of Escherichia coli, in E coli and Salmonella typhimurium, Cellular and Molecular Biology, 2nd ed (Neidhardt, F. C., Curtiss, III, R., Ingraham, J. L., Lin, E. C C., Low, K. B., Magasanik, B., Regnikoff, W. S., Riley, M., Schaechter, M., and Umbarger, H. E., eds.), ASM, pp. 2460–2488.
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© 1997 Humana Press Inc., Totowa, NJ
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Labigne, A. (1997). Random Mutagenesis of the H. pylori Genome. In: Clayton, C.L., Mobley, H.L.T. (eds) Helicobacter pylori Protocols. Methods in Molecular Medicine, vol 8. Humana Press. https://doi.org/10.1385/0-89603-381-3:153
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DOI: https://doi.org/10.1385/0-89603-381-3:153
Publisher Name: Humana Press
Print ISBN: 978-0-89603-381-8
Online ISBN: 978-1-59259-592-1
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