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The Nonhuman Primate Model for H. pylori Infection

  • Andre Dubois
  • Douglas E. Berg
Part of the Methods in Molecular Medicine book series (MIMM, volume 8)

Abstract

Helicobacter pylori establishes chronic infections in the human gastric mucosa that can last for decades, and that are a major cause of gastritis and peptic ulcer disease (PUD) and a risk factor for gastric cancer. The importance of H. pylori as a human pathogen has led to major efforts to understand its interactions with its human host, including the development and use of various animal models. Several heterologous models have been developed, including:
  1. 1.

    Gnotobiotic newborn piglets, which are easily infected by H. pylori of human origin (1,2), but are best for short term studies lasting no more than about a month,

     
  2. 2.

    Mice and ferrets, which can be colonized for months and years, respectively, although most easily by Helicobacter species other than H. pylori (3,4),

     
  3. 3.

    Particular strains of mice, which can be colonized by selected H. pylori strains (56); and

     
  4. 4.

    Domestic cats, which can also carry H. pylori (7), but which exhibit an inflammatory response different from that seen in humans (mononuclear infiltration deep near the lamina propria, not throughout the mucosa) and which also differ in diet and gastric anatomy

     

Keywords

Rhesus Monkey Pylorus Infection Nonhuman Primate Pylorus Strain Brain Heart Infusion Broth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Krakowka, S., Morgan, D. R., Kraft, W. G., and Leunk, R D (1987) Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect. Immun. 55, 2789–2796.PubMedGoogle Scholar
  2. 2.
    Akopyants, N. S., Eaton, K. A., and Berg, D. E. (1995) Adaptive mutation and cocolonization during Helicobacter pylori infection of gnotobiotic piglets. Infect. Immun. 63, 116–121.PubMedGoogle Scholar
  3. 3.
    Lee A., Fox, J. G., Otto, G., and Murphy, J. (1990) A small animal model of human Helicobacter pyloi active chronic gastritis. Gastroenterology 99, 1315–1323PubMedGoogle Scholar
  4. 4.
    Fox, J. G., Otto, G., Taylor, N. S., Rosenblad, W., and Murphy, J. C (1991) Helicobacter mustelae-induced gastritis and elevated gastric pH in the ferret (Mustela putorius furo). Infect Immun. 59, 1875–1880PubMedGoogle Scholar
  5. 5.
    Marchetti, M., Arico, B., Burroni, D., Figura, N., Rappuoli, R., and Ghiara, P. (1995) Development of a mouse model of Helicobacter pylori infection that mimics human disease. Science 267, 1655–1658.PubMedCrossRefGoogle Scholar
  6. 6.
    McColm, A. A., Bagshaw, J. O’Malley C., and McLaren, A (1995) Development of a mouse model of gastric colonisation with Helicobacter pylori. Gut 37(Suppl 1), 198(abstract).Google Scholar
  7. 7.
    Handt, L. K., Fox, J. O., Dewhirst, F. E., Fraser, G. J., Paster, B. J., Yan, L. L., Rozmiarek, H., Rufo, R., and Stalis, I. H. (1994) Helicobacter pylori isolated from the domestic cat: public health implications. Infect. Immun 62, 2367–2374.PubMedGoogle Scholar
  8. 8.
    Newell, D. G., Hudson, M J., and Baskerville, A. (1987) Naturally occurring gastritis associated with Campylobacter pylori infection in the Rhesus monkey. Lancet ii, 1338.CrossRefGoogle Scholar
  9. 9.
    Curry, A., Jones, D. M., and Eldridge, J (1987) Spiral organisms in the baboon stomach Lancet ii, 634,635CrossRefGoogle Scholar
  10. 10.
    Bronsdon, M. A. and Schoenknecht, F D (1988) Campylobacter pylori isolated from the stomach of the monkey Macaca nemestrina. J. Clin Microbiol 26, 1725–1728.PubMedGoogle Scholar
  11. 11.
    Dubois, A., Tarnawski, A., Newell, D. G., Fiala, N., Dabros, W., Stachura, J, Krivan, H., and Heman-Ackah, L M (1991) Gastric injury and invasion of parietal cells by spiral bacteria in rhesus monkeys. Are gastritis and hyperchlorhydria infectious diseases? Gastroenterology 100, 884–891PubMedGoogle Scholar
  12. 12.
    Dubois, A., Fiala, N., Heman-Ackah, L. M., Drazek, E S., Tarnawski, A, Fishbein, W. N, Perez-Perez, G I, and Blaser, M. J (1994) Natural gastric infection with Helicobacter pylori in monkeys. A model for human infection with spiral bacteria. Gastroenterology 106, 1405–1417.PubMedGoogle Scholar
  13. 13.
    Hazell, S. L, Eichberg, J. W., Lee, D. R., Alpert, L., Evans, D G., Evans, D. J., Jr., and Graham, D. Y. (1992) Selection of the chimpanzee over the baboon as a model for Helicobacter pylori infection Gastroenterology 103, 848–854.PubMedGoogle Scholar
  14. 14.
    Bronsdon, M. A., Goodwin, C. S., Sly, L. I., Chilvers, T, and Schoenknecht, F D. (1991) Helicobacter nemestrinae sp. nav., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina). Int. J. Syst. Bacteriol. 41, 148–153.PubMedCrossRefGoogle Scholar
  15. 15.
    Drazek, E. S, Dubois, A., and Holmes, R. K. (1994) Characterization and presumptive identification of Helicobacter pylori isolates from rhesus monkeys J Clin. Microbial 32, 1799–1804.Google Scholar
  16. 15a.
    Romaniuk, P. J., Zoltowska, B, Trust, T. J, Lane, D. J., Olsen, G J., Pace, N R., and Stahl, D. A. (1987) Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp J Bacteriol 169, 2137–2141Google Scholar
  17. 16.
    Shuto, R., Fujioka, T., Kubota, T, and Nasu, M. (1993) Experimental gastritis induced by Helicobacter pylori in Japanese monkeys Infect. Immun. 61, 933–939PubMedGoogle Scholar
  18. 17.
    Euler, A. R., Zurenko, G. E., Moe, J. B., Ulrich, R. G., and Yagi, Y. (1990) Evaluation of two monkey species (Macaca mulatta and Macaca fascicularis) as possible model for human Helicobacter pyloridisease J Clin Microbiol 28, 2285–2290.PubMedGoogle Scholar
  19. 18.
    Napier, J. R. and Napier, P H. (1967) A Handbook of Living Primates Morphology, Ecology and Behavior of Non-Human Primates. Academic, New York, NYGoogle Scholar
  20. 19.
    Dubois, A., Nompleggi, D., and Castell, D 0. (1988) Histamine H2 receptor stimulation increases gastric emptying in monkeys. Am J Physiol. 255, G767–G771.PubMedGoogle Scholar
  21. 20.
    Srinivasan, V. and Dubois, A (1995) Use of non-human primates for studies of gastrointestinal diseases, in Essentials of Experimental Surgery Gastroenterology (Moody, F., ed.), Harwood Academic, Reading, UK, 1995; Chapter 27, pp. 1–19.Google Scholar
  22. 21.
    Parker, G. A., Gilmore, C. J, and Dubois, A. (1981) Spontaneous gastric ulcer in a rhesus Monkey Brain Res Bull 6, 445–447.PubMedCrossRefGoogle Scholar
  23. 22.
    Natelson, B. J., Dubois, A, and Sodetz, F. J. (1977) Effect of multiple stress procedures on monkey gastroduodenal mucosa, serum gastrin and hydrogen ion kinetics. Am J Dig Des 22, 888–897.CrossRefGoogle Scholar
  24. 23.
    O’Gara, R. W. and Adamson, R. H. (1972) Spontaneous and induced neoplasms in nonhuman primates, in Pathology of Human Primates Part I (Fiennes, R. L., ed.), Basel, Karger, pp. 190–238.Google Scholar
  25. 24.
    Bruley des Varannes, S., Mizrahi, M., and Dubois, A. (1991) Relation between postprandial gastric emptying and cutaneous electrogastrogram in primates. Am J Physiol 261(Gastrointestinal Liver Physiol. 24), 248–255Google Scholar
  26. 25.
    Dubois, A., Berg, D. E., Incecik, E T., Fiala, N., Heman-Ackah, L. M., Perez-Perez, G. I., and Blaser, M. J (1996) Transient and persistent experimental infection of non-human primates with Helicobacter pylori. implications for human disease Inf Immunol. 64, 2885–2891.Google Scholar
  27. 26.
    Andersen, L. P., Norgaard, A., Holck, S., Blom, J., and Elsborg, L (1996) Isolation of a “Helicobacter heilmannii”-like organism from the human stomach. Eur J. Clin. Microbiol. Inf. Dis 15, 95,96.CrossRefGoogle Scholar
  28. 27.
    Solnick, J. V., O’Rourke, J., Lee, A., and Tompkins, L. S. (1993) Molecular analysis of urease genes from a newly identified uncultured species of. Helicobacter J Inf. Dis. 168, 379–383.Google Scholar
  29. 28.
    Mendes, E. N., Queiroz, D. M., Dewhirst, F. E., Paster, B J., Carvahlo, S. D, Magnano, A. G. P., Moura, S. B., Rocha, G. A, and Olivera, A M R. (1995) Reservoir hosts for Gastrospirillum infection. Gut 37(Suppl 1), A26 (abstract 104)Google Scholar
  30. 29.
    Dubois, A., Fiala, N., Weichbrod, R. H., Ward, G. S., Nix, M., Mehlman, P. T., Taub, D M., Perez-Perez, G I., and Blaser, M. J. (1995) Seroepizootiology of H. pylori gastric infection in socially-housed rhesus monkeys. J. Clin. Microbiol. 33, 1492–1495.PubMedGoogle Scholar
  31. 30.
    Cover, T. L. and Blaser, M. J. (1994) Purification and characterization of the vacuolating toxin from Helicobacter pylori J. Biol. Chem 267, 10,570–10,575.Google Scholar
  32. 31.
    Tummuru, M. K., Cover, T. L., and Blaser, M. J. (1993) Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production Infect. Immun 61, 799–809.Google Scholar
  33. 32.
    Perez-Perez, G. I., Dworkin, B. M., Chodos, J., E., and Blaser, M J. (1988) Campylobacter pylori antibodies in humans Ann Int. Med 109, 11–17PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1997

Authors and Affiliations

  • Andre Dubois
    • 1
  • Douglas E. Berg
    • 2
  1. 1.Department of PathologyUniformed Services University of the Health SciencesBethesda
  2. 2.Department of Molecular MicrobiologyWashington University Medical SchoolSt. Louis

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