Molecular Methods for Detecting Ulcerogenic Strains of H. pylori

  • John C. Atherton
Part of the Methods in Molecular Medicine book series (MIMM, volume 8)


Certain nonconserved genotypic and phenotypic characteristics of H. pylori are associated with increased risk of peptic ulceration in the human host. These characteristics can be divided into two groups: first, those relating to vacuolating cytotoxin activity (1,2), and differences in the gene encoding the cytotoxin, vacA (3); second, those relating to the cytotoxin-associated gene, cagA (4,5).


Colony Hybridization cagA Typing vacA Type Klenow Enzyme Ulcer Risk 
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  1. 1.
    Figura, N, Guglielmetti, P, Rossolini, A, Barberi, A, Cusi, G, Musmanno, R A, Russi, M, and Quaranta, S (1989) Cytotoxin production by Campylobacterpylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only. J Clin Microbiol. 27, 225–226PubMedGoogle Scholar
  2. 2.
    Rautelin, H., Blomberg, B., Jarnerot, G, and Danielsson, D (1994) Nonopsonic activation of neutrophils and cytotoxin production by Helicobacter pylori-ulcerogenic markers. Scand J Gastroenterol. 29, 128–132.PubMedCrossRefGoogle Scholar
  3. 3.
    Atherton, J C, Cao, P, Peek, R. M., Jr., Tummuru, M. K. R., Blaser, M J., and Cover, T. L. (1995) Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori: association of specific vacA types with cytotoxin production and peptic ulceration. J. Biol Chem 270, 17,771–l7,777PubMedCrossRefGoogle Scholar
  4. 4.
    Cover, T L., Dooley, C P., and Blaser, M J. (1990) Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect. Immun 58, 603–610.PubMedGoogle Scholar
  5. 5.
    Crabtree, J. E, Taylor, J. D., Wyatt, J. I., Heatley, R V., Shallcross, T M, Tompkins, D. S., and Rathbone, B. J. (1991) Mucosal IgA recognition of Helicbacter pylori 120 kDa protein, peptic ulceration, and gastric pathology. Lancet 338, 332–335PubMedCrossRefGoogle Scholar
  6. 6.
    Tummuru, M. K. R., Sharma, S. A., and Blaser, M. J. (1995) Helicobacter pylori picB, a homolog of the Bordtella pertussis toxin secretion protein, is required for induction of IL-8 in gastric epithelial cells. Mol Microbiol. 18, 867–876PubMedCrossRefGoogle Scholar
  7. 7.
    Atherton, J. C., Peek, R. M., Tham, K. T., Blaser, M. J., and Cover, T L. (1996) Clinical and pathological importance of heterogeneity in vacA. The vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterol, in press.Google Scholar
  8. 8.
    Hawrylik, S. J., Wasilko, D. J., Haskell, S. L., Gootz, T. D., and Lee, S E. (1994) Bisulfite or sulfite inhibits growth of Helicobacter pylori. J. Clin. Microbiol. 32, 790–792PubMedGoogle Scholar
  9. 9.
    Cover, T. L., Tummuru, M. K. R., Cao, P., Thompson, S. A., and Blaser, M J. (1994) Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J Biol. Chem. 269, 10,566–10,573PubMedGoogle Scholar
  10. 10.
    Tummuru, M. K. R., Cover, T. L., and Blaser, M. J. (1993) Cloning and expression of a high molecular weight major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production. Infect Immun 61, 1799–1809.PubMedGoogle Scholar
  11. 11.
    Kwok, S. (1990) Procedures to minimize PCR-product carry-over, and Orrego, C. (1990) Organizing a laboratory for PCR work, in PCR Protocols. A Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Sninsky, J. J, and White, T. J, eds.), Academic, San Diego, CA, pp. 142–145 and 447-454.Google Scholar
  12. 12.
    Feinberg, A. P. and Vogelstein, B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 132, 6–13.PubMedCrossRefGoogle Scholar
  13. 13.
    Feinberg, A P. and Vogelstein, B. (1984) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal.Biochem 137, 266,267.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1997

Authors and Affiliations

  • John C. Atherton
    • 1
  1. 1.Division of Gastroenterology and Institute of Infections and ImmunityUniversity HospitalNottinghamUK

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