Abstract
Development of the technique of in situ hybridization (ISH) propelled the merger of the fields of molecular genetics and cytogenetics. Refinement of the technique through the use of fluorescence (FISH) produced a remarkably powerful research and diagnostic tool.
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References
Gall J.G and Pardue M. L (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Nat1 Acad Sci USA 63, 378ā383
Pardue M. L and Gall J. G. (1970) Science 168, 1356
Gerhard D S, Kawasaki E S, Bancroft F. C, and Szabo P. (1981) Localization of a unique gene by direct hybridization in situ Proc Nat1 Acad. Scl USA 78, 3755ā3759
Harper M. E. and Saunders G. F (1981) Locahzation of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83, 43lā439.
Langer-Safer P R, Levine M., and Ward D C. (1982) Immunological method for mapping genes on Drosophila polytene chromosomes Proc. Natl Acad Sci USA 79, 438lā4385
Bhatt B., Burns J., Flannery D, and McGee J 0. (1988) Direct visualization of single copy genes on banded metaphase chromosomes by nonisotopic in situ hybridization Nucl Acids Res 16, 395lā3961.
Lawrence J. B, Villnave C. A, and Singer R H. (1988) Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell 52, 51ā61.
Lichter P. (1990) High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247, 61.
Lawrence J B, Singer R H, and McNeil J. A (1990) Interphase and metaphase resolution of different distances within the human dystrophin gene Science 249, 928
Koch J, HindkJaer J., Mogensen J., Kolvraa S., and Bolund L (1991) An improved method for chromosome-specific labeling of alpha satellite DNA in situ by using denatured double-stranded DNA probes as primers in a primed in situ labeling procedure GATA 8, 171ā178
Lengauer C., Eckelt A, Weith A, Endlich N., Ponelies N., Lichter P, Gruelich K. 0, and Cremer T (1991). Painting of defined chromosomal regions by in situ suppression hybridization of libraries from laser-microdissected chromosomes Cytogenet Cell Genet 56, 27ā30.
Telemus H., Pelmear A H. P, Tunnacliffe A., Carter N. P, Behmel A, Ferguson-Smith M. A, NordenskJold M, Prfagner R, and Ponder B (1992). Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes Genes Chrom. Cancer 4, 257ā263.
Komminoth P and Long A. A. (1995) Review: In situ polymerase chain reactionāmethodology, applications, and nonspecific pathways, in PCR Applications Manual, Boehringer Mannheim GmbH, Biochemica, Germany, 97ā112
NUOVO G. J. (1994) PCR In Situ Hybridization Protocols and Applications, 2nd ed., Raven Press, Ltd., New York.
Briley G. P, Riggs P. K, Womack J E, Hancock D L., and Bidwell C A (1996). Chromosomal localization of the porcine skeletal muscle calpain gene, Mamm Genome 7, 226ā228
Neibergs H. L., Gallagher D S., Georges M., Sargeant L. S., Dietz A. B., and Womack J E. (1993) Physical mapping of inhibin-beta-A in domestic cattle Mamm. Genome 4, 328ā332
Pinkel D, Straume T., and Gray J. W (1986) Cytogenetic analysis using quantitative, high-sensitivity fluorescence hybridization Proc Natl. Acad Sci USA 83, 2934ā2938.
Brandiff B F, Gordon L A, and Trask B J. (1991) DNA sequence mapping by fluorescence in situ hybridization Env Mol Mutagen. 18, 259ā262
Chrisman C. L, Briley G P, and Waldbieser G C. (1991) In situ hybridization and high-resolution banding of chromosomes, in Gene Mapping Techniques and Applications (Schook L B, Lewin H. A, and McLaren D G, eds. ), Marcel Dekker, Inc, New York.
Dyer K. and Meyne J. (1991) Molecular cytogenetics Use of DNA probes as an adjunct to classical clinical cytogenetics, in The ACT Cytogenetics Laboratory Manual, 2nd ed. (Barth M J, ed.), Raven, New York, 525ā537
Lichter P, Boyle A, Cremer T, and Ward D C (1991). Analysis of genes and chromosomes by nonisotopic in situ hybridization GATA 8, 24ā35
Trask B J (1991) Fluorescence in situ hybridization applications in cytogenetics and gene mapping Trends Genet 7, 149ā154
Lemteux N, Dutrillaux B, and Viegas-Pequignot E (1992) A simple method for simultaneous R-or G-banding and fluorescence in situ hybridization of small single-copy genes Cytogen Cell Genet 59, 311,312
Weigant J, Wiesmeijer C C, Hoovers J M N, Schuuring E, dāAzzo A, Vrolyk J, Tanke H. J, and Raap A. K. (1993) Multiple and sensitive fluorescence in situ hybridization with rhodamme-, fluorescein-and coumarin-labeled DNAs Cytogenet Cell Genet. 63, 73ā76
Mechanic M (1988) Biotinylated DNA probes powerful and practical tools for visualization of complementary nucleic acids on Southern blots or in situ Immunochemica 2, 12ā14
Florijn R J, Slats J, Tanke H J, and Raap A. K. (1995) Analysis of antifading reagents for fluorescence microscopy. Cytometry 19, 177ā182
Johnson G D. and Nogueira-Araujo G. M (1981) A simple method of reducing the fading of immunofluorescence during microscopy J Immunol Methods 43, 349,350
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Riggs, P.K., Hahn, K.A. (1998). Fluorescence In Situ Hybridization to Chromosomes. In: Hanausek, M., Walaszek, Z. (eds) Tumor Marker Protocols. Methods in Molecular Medicineā¢, vol 14. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-380-5:245
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DOI: https://doi.org/10.1385/0-89603-380-5:245
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