Abstract
The epitopes recognized by the antigen receptors on T-lymphocytes (T-cells) consist of a molecular complex made up of a self-protein and a short linear peptide (1). The self protein is a product of the major histocompatibility gene complex (MHC), and is classified as either a Class I or a Class II molecule. Class I and Class II MHC molecules are specialized to hold and present peptides on the surface of the cell, making them available for interaction with the T-cell receptor (TCR). Despite the structural similarity of Class I and Class II MHC molecules, their functional roles are quite different. Class I MHC molecules are present on most cells in the body and are specialized for presentation of peptides derived from proteins synthesized within the cell. These Class I-peptide complexes are recognized by cytotoxic T-cells (Tc or CTL), and are corecognized by the CD8 molecules present on the surface of CD8+ cells. In contrast, Class II MHC molecules are present only on certain specialized antigen-presenting cells (APC) and are recognized by helper T-cells (Th), which carry the CD4 molecule as the corecognition molecule for MHC Class II. As the names imply, Tc generally take the role of killer cells on recognition of an MHC Class I-peptide complex, whereas Th are programmed to help both the antibody and cytotoxic T-cell responses following contact with their specific MHC Class II-peptide complex.
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References
Brown, J. H., Jardetzky, T. S., Gorga, J. C., Stern, L. J., Urban, R. G., Strominger, J. L., and Wiley, D. C. (1993) Three-dimensional structure of the human class II histocompatibility antigen HLA-DRI. Nature 364, 33–39.
Suhrbier, A., Rodda, S. J., Ho, P. C., Csurhes, P., Dunckley, H., Saul, A., Geysen, H. M., and Rzepczyk, C. M. (1991) Role of single amino acids in the recognition of a T cell epitope. J. Immunol. 147, 2507–2513.
Geysen, H. M., Rodda, S. J., Mason, T. J., Tribbick, G., and Schoofs, P. G. (1987) Strategies for epitope analysis using peptide synthesis. J. Immunol. Methods 102, 259–274.
Mutch, D. A., Rodda, S. J., Benstead, M., Valerio, R. M., and Geysen, H. M. (1991) Effects of end groups on the stimulatory capacity of minimal length T cell determinant peptides. Pept. Res. 4, 132–137.
Brett, S. J., Blau, J., Hughes-Jenkins, C. M., Rhodes, J., Liew, F. Y., and Tote, J. P. (1991) Human T cell recognition of influenza A nucleoprotein: Specificity and genetic restriction of immunodominant T helper cell epitopes. J. Immunol. 147, 984–991.
Via, C. S., Tsokos, G. C., Stocks, N. I., Clenci, M., and Shearer, G. M. (1990) Human in vitro allogeneic responses: Demonstration of three pathways of T helper activation. J. Immunol. 144, 2524–2528.
Gammon, G., Klotz, J., Ando, D., and Sercarz, E. E. (1990) The T cell repertoire to a multideterminant antigen: Clonal heterogeneity of the T cell response, variation between syngeneic individuals, and in vitro selection of T cell specificities. J. Immunol. 144, 1571–1577.
Fard, G. A. B. P., Latchman, Y., Rodda, S., Geysen, M., Roitt, I., and Brostoff, J. (1993) T cell epitopes of the major fraction of rye grass Lolium perenne (Lol pI) defined using overlapping peptides in vitro and in vivo. I: Isoallergen clone 1A. Clin. Exp. Immunol. 94, 111–116.
Reece, J. C., Geysen, H. M., and Rodda, S. J. (1993) Mapping the major human T helper epitopes of tetanus toxin: The emerging picture. J. Immunol. 151, 6175–6184.
Reece, J. C., McGregor, D. L., Geysen, H. M., and Rodda, S. J. (1994) Scanning for T helper epitopes with human PBMC using pools of short synthetic peptides. J. Immunol. Methods 172, 241–254.
Mutch, D. A., Underwood, J. R., Geysen, H. M., and Rodda, S. J. (1994) Comprehensive T cell epitope mapping of HIV-1-env antigens reveals many areas recognized by HIV-1-seropositive and by low risk HIV-1-seronegative individuals. J. AIDS 7, 879–890.
Jung, G. and Beck-Sickinger, A. (1992) Multiple peptide synthesis methods and their applications. Angew. Chem. 31, 367–383.
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© 1996 Humana Press Inc.
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Rodda, S.J. (1996). T-Cell Epitope Mapping with Synthetic Peptides and Peripheral Blood Mononuclear Cells. In: Morris, G.E. (eds) Epitope Mapping Protocols. Methods in Molecular Biology™, vol 66. Humana Press. https://doi.org/10.1385/0-89603-375-9:363
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DOI: https://doi.org/10.1385/0-89603-375-9:363
Publisher Name: Humana Press
Print ISBN: 978-0-89603-375-7
Online ISBN: 978-1-59259-552-5
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