Abstract
SPOT synthesis is an easy and very flexible technique for simultaneous parallel chemical synthesis on membrane supports. This method gives immunologists rapid and low-cost access to large numbers of peptides both as solid-phase-bound and solution-phase products for systematic epitope analysis.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Frank, R., Güler, S., Krause, S., and Lindenmaier, W. (1991) Facile and rapid “spot-synthesis” of large numbers of peptides on membrane sheets, in Peptides 1990 (Giralt, E. and Andreu, D., eds.), ESCOM Science Publishers B. V., Leiden, pp. 151, 152.
Frank, R. (1992) Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48, 9217–9232.
Krchnák, V., Vágner, J., Safár, P., and Lebl, M. (1988) Noninvasive continuous monitoring of solid phase peptide synthesis by acid-base indicator. Collect. Czech. Chem. Commun. 53, 2542–2548.
Kramer, A., Volkmer-Engert, R., Malin, R., Reineke, U., and Schneider-Mergener, J. (1993) Simultaneous synthesis of peptide libraries on single resin and continuous cellulose membrane supports: examples for the identification of protein, metal and DNA binding peptide mixtures. Pept. Res. 6, 314–319.
Frank, R. (1994) Spot-synthesis: an easy and flexible tool to study molecular recognition, in Innovations and Perspectives in Solid Phase Synthesis 1994 (Epton, R., ed.), Mayflower Worldwide Ltd., Birmingham, pp. 509–512.
Bray, A. M., Maeji, N. J., and Geysen, H. M. (1990) The simultaneous multiple production of solution phase peptides; Assessment of the Geysen method of simultaneous peptide synthesis. Tetrahedron Lett. 31, 5811–5814.
Hoffmann, S. and Frank, R. (1994) A new safety-catch peptide-resin linkage for the direct release of peptides into aqueous buffers. Tetrahedron Lett. 35, 7763–7766.
Hoffmann, S. and Frank, R. (1995) in preparation.
Kramer, A., Schuster, A., Reineke, U., Malin, R., Volkmer-Engert, R., Landgraf, C., and Schneider-Mergener, J. (1994) Combinatorial cellulose-bound peptide libraries: screening tools for the identification of peptides that bind ligands with predefined specificity. METHODS (Comp. Methods Enzymol.) 6, 912–921.
Geysen, H. M., Rodda, S. J., and Mason, T. J. (1986) A priori delineation of a peptide which mimics a discontinuous antigenic determinant. Mol. Immunol. 23, 709–715.
Dooley, C. T. and Houghten, R. A. (1993) The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands. Life Sci. 52, 1509–1517.
Frank, R., Kieb, M., Lahmann, H., Behn, Ch., and Gausepohl, H. (1995) Combinatorial synthesis on membrane supports by the SPOT technique, in Peptides 1994 (Mara, H. L. S., en.), ESCOM, Leiden, pp. 479, 480.
Ersfeld, K., Wehland, J., Plessmann, U., Dodemont, H., Gerke, V., and Weber, K. (1993) Characterization of the tubulin-tyrosine ligase. J. Cell Biol. 120, 725–732.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Humana Press Inc.
About this protocol
Cite this protocol
Frank, R., Overwin, H. (1996). SPOT Synthesis. In: Morris, G.E. (eds) Epitope Mapping Protocols. Methods in Molecular Biology™, vol 66. Humana Press. https://doi.org/10.1385/0-89603-375-9:149
Download citation
DOI: https://doi.org/10.1385/0-89603-375-9:149
Publisher Name: Humana Press
Print ISBN: 978-0-89603-375-7
Online ISBN: 978-1-59259-552-5
eBook Packages: Springer Protocols