Advertisement

SPOT Synthesis

Epitope Analysis with Arrays of Synthetic Peptides Prepared on Cellulose Membranes
  • Ronald Frank
  • Heike Overwin
Part of the Methods in Molecular Biology™ book series (MIMB, volume 66)

Abstract

SPOT synthesis is an easy and very flexible technique for simultaneous parallel chemical synthesis on membrane supports. This method gives immunologists rapid and low-cost access to large numbers of peptides both as solid-phase-bound and solution-phase products for systematic epitope analysis.

Keywords

Amino Acid Solution Peptide Pool Membrane Support Peptide Assembly Fmoc Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Frank, R., Güler, S., Krause, S., and Lindenmaier, W. (1991) Facile and rapid “spot-synthesis” of large numbers of peptides on membrane sheets, in Peptides 1990 (Giralt, E. and Andreu, D., eds.), ESCOM Science Publishers B. V., Leiden, pp. 151, 152.Google Scholar
  2. 2.
    Frank, R. (1992) Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48, 9217–9232.CrossRefGoogle Scholar
  3. 3.
    Krchnák, V., Vágner, J., Safár, P., and Lebl, M. (1988) Noninvasive continuous monitoring of solid phase peptide synthesis by acid-base indicator. Collect. Czech. Chem. Commun. 53, 2542–2548.CrossRefGoogle Scholar
  4. 4.
    Kramer, A., Volkmer-Engert, R., Malin, R., Reineke, U., and Schneider-Mergener, J. (1993) Simultaneous synthesis of peptide libraries on single resin and continuous cellulose membrane supports: examples for the identification of protein, metal and DNA binding peptide mixtures. Pept. Res. 6, 314–319.PubMedGoogle Scholar
  5. 5.
    Frank, R. (1994) Spot-synthesis: an easy and flexible tool to study molecular recognition, in Innovations and Perspectives in Solid Phase Synthesis 1994 (Epton, R., ed.), Mayflower Worldwide Ltd., Birmingham, pp. 509–512.Google Scholar
  6. 6.
    Bray, A. M., Maeji, N. J., and Geysen, H. M. (1990) The simultaneous multiple production of solution phase peptides; Assessment of the Geysen method of simultaneous peptide synthesis. Tetrahedron Lett. 31, 5811–5814.CrossRefGoogle Scholar
  7. 7.
    Hoffmann, S. and Frank, R. (1994) A new safety-catch peptide-resin linkage for the direct release of peptides into aqueous buffers. Tetrahedron Lett. 35, 7763–7766.Google Scholar
  8. 8.
    Hoffmann, S. and Frank, R. (1995) in preparation.Google Scholar
  9. 9.
    Kramer, A., Schuster, A., Reineke, U., Malin, R., Volkmer-Engert, R., Landgraf, C., and Schneider-Mergener, J. (1994) Combinatorial cellulose-bound peptide libraries: screening tools for the identification of peptides that bind ligands with predefined specificity. METHODS (Comp. Methods Enzymol.) 6, 912–921.Google Scholar
  10. 10.
    Geysen, H. M., Rodda, S. J., and Mason, T. J. (1986) A priori delineation of a peptide which mimics a discontinuous antigenic determinant. Mol. Immunol. 23, 709–715.PubMedCrossRefGoogle Scholar
  11. 11.
    Dooley, C. T. and Houghten, R. A. (1993) The use of positional scanning synthetic peptide combinatorial libraries for the rapid determination of opioid receptor ligands. Life Sci. 52, 1509–1517.PubMedCrossRefGoogle Scholar
  12. 12.
    Frank, R., Kieb, M., Lahmann, H., Behn, Ch., and Gausepohl, H. (1995) Combinatorial synthesis on membrane supports by the SPOT technique, in Peptides 1994 (Mara, H. L. S., en.), ESCOM, Leiden, pp. 479, 480.Google Scholar
  13. 13.
    Ersfeld, K., Wehland, J., Plessmann, U., Dodemont, H., Gerke, V., and Weber, K. (1993) Characterization of the tubulin-tyrosine ligase. J. Cell Biol. 120, 725–732.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Ronald Frank
    • 1
  • Heike Overwin
    • 1
  1. 1.Gesellschaft für Biotechnoligische Forschung mbHBraunschweigGermany

Personalised recommendations