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Quantitation of HIV-1 RNA in Genital Secretions

  • Protocol
HIV Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 17))

Abstract

The continuing spread of the HIV-1 epidemic worldwide has stimulated efforts to develop vaccines and decrease transmission of HIV-1 as noted in Chapter 8. Key to this effort is the characterization of the qualities of HIV-1 in genital secretions. Seminal cell and seminal plasma culture have been used to isolate HIV-1 from semen but have low recovery rates of 9–50% (1–11). Culture of vaginal cells have also had low recovery rates of 0–30% (12–14). In an attempt to overcome this problem, HIV-1 has been evaluated in genital secretions using polymerase chain reaction (PCR) techniques. Compared to culture techniques, PCR techniques, both DNA and RNA, have had a higher recovery rate of HIV-1 from genital secretions (2,3,7,9–11,13–25).

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Author information

Authors and Affiliations

  1. Henry M. Jackson Foundation, Rockville, MD

    Bruce L. Gilliam

Authors
  1. Bruce L. Gilliam
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Editor information

Editors and Affiliations

  1. Walter Reed Army Institute of Research, Rockville, MD

    Nelson L. Michael MD, PhD

  2. Henry M. Jackson Foundation, Rockville, MD

    Jerome H. Kim MD

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© 1999 Humana Press Inc., Totowa, NJ

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Gilliam, B.L. (1999). Quantitation of HIV-1 RNA in Genital Secretions. In: Michael, N.L., Kim, J.H. (eds) HIV Protocols. Methods in Molecular Medicine™, vol 17. Humana Press. https://doi.org/10.1385/0-89603-369-4:151

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  • DOI: https://doi.org/10.1385/0-89603-369-4:151

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-369-6

  • Online ISBN: 978-1-59259-601-0

  • eBook Packages: Springer Protocols

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